Mouse IL-7R alpha/CD127 Alexa Fluor® 700-conjugated Antibody
Mouse IL-7R alpha/CD127 Alexa Fluor® 700-conjugated Antibody Summary
Glu21-Asp239
Accession # P16872
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
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Preparation and Storage
Background: IL-7R alpha/CD127
Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature mouse IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type III domain and a WSxWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Within the ECD, mouse IL-7 R alpha shares 67% and 79% aa sequence identity with human and rat IL‑7 R alpha, respectively. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL-7 show cross-species activity through the IL-7 receptor (3, 5). IL‑7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL‑7 R alpha is critical for both T cell and B cell lineage development (8). In human it is required for T cell but not for B cell development (9). IL‑7 induces the down regulation and shedding of cell surface IL-7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL‑7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL‑7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).
- Mazzucchelli, R. and S.K. Durum, 2007, Nat. Rev. Immunol. 7:144.
- Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.
- Goodwin, R.G. et al. (1990) Cell 60:941.
- Noguchi, M. et al. (1993) Science 262:1877.
- Barata, J.T. et al. (2006), Exp. Hematol. 34:1133.
- Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
- Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
- Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
- Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
- Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
- Park, L.S. et al. (2000) J. Exp. Med. 192:659.
- Pandey, A. et al. (2000) Nat. Immunol. 1:59.
- Reche, P.A. et al. (2001) J. Immunol. 167:336.
- Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
- Jahn, T. et al. (2007) Blood 110:1840.
- Lai, L. et al. (2006) Blood 107:1776.
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Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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