Mouse IL-9 Antibody

Catalog #: AF409
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF409
AF409-SP

Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody.

1 Image

All IL-9 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-9 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with human IL-9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑9
Gln19-Pro144 (Predicted)
Accession # P15247

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑9 (Catalog # 409-ML)

Neutralization

Measured by its ability to neutralize IL‑9-induced proliferation in the TS1 mouse helper T cell line. Moreau, F. J. et al. (1988) Nature 336:690. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 0.05 ng/mL Recombinant Mouse IL‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

View Larger

Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody. Recombinant Mouse IL‑9 (Catalog # 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF409). The ND₅₀ is typically 0.5-1.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9

Mouse IL-9 was originally identified as a T cell-derived T cell growth factor III/P40 which could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. IL-9 can also prolong the in vitro survival of other T cell clones as well as potentiate the IL-2 dependent proliferation of mouse fetal thymocytes. However, this cytokine has no growth-stimulating activity on mouse cytolytic T cell clones or fresh T cells. In addition to its activities on T cells, mouse IL-9 also has mast cell enhancing activity (MEA) and can enhance the mIL-3- or mIL-4-dependent proliferation of mouse bone marrow-derived mast cells. Furthermore, IL-9 will synergize with erythropoietin to support erythroid colony formation in vitro.

The gene for mIL-9 has been mapped to mouse chromosome 13. The mouse IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Mouse IL-9 contains four potential N-linked glycosylation sites and the native mIL-9 is a highly glycosylated protein.

Human IL-9 was independently cloned as a novel growth factor which is mitogenic for the human megakaryoblastic leukemic cell line, M07e. Human and mouse IL-9 share 56% and 67% homology at the amino acid and nucleotide levels, respectively. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells.

References

Renauld, J.E. et al. (1995) J. Leukoc. Biol 57:303.

Long Name

Interleukin 9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Alternate Names

Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse IL-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Involvement of IL-9 in Th17-associated inflammation and angiogenesis of psoriasis.
Authors: Singh T, Schon M, Wallbrecht K, Gruber-Wackernagel A, Wang X, Wolf P
PLoS ONE, 2013-01-15;8(1):e51752.
Species: Mouse
Sample Types: In Vivo, Whole Tissue
Applications: IHC-P, Neutralization