Mouse Integrin alpha E/CD103 Biotinylated Antibody

Catalog #: BAF1990 Datasheet / COA / SDS

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BAF1990

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Mouse Integrin alpha E/CD103 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha E/CD103 in Western blots. In Western blots, less than 2% cross‑reactivity with recombinant mouse (rm) Integrin alpha 2, rmIntegrin alpha 3, rmIntegrin alpha 4, rmIntegrin alpha M, and recombinant human Integrin alpha V is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha E
Phe20-Arg1113 (Ser453Gly)
Accession # ABD49099

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Integrin alpha E/CD103

Flow Cytometry

2.5 µg/10⁶ cells

Mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha E/CD103

The alpha E subunit, also known as CD103, forms a non-covalent heterodimer with Integrin beta 7. Integrin alpha E/ beta 7 is a receptor for E-Cadherin. Integrin alpha E is expressed in intra‑epithelial lymphocytes and mediates adhesion of intra‑epithelial lymphocytes to epithelial cell monolayers.

Entrez Gene IDs

3682 (Human); 16407 (Mouse); 83577 (Rat)

Alternate Names

antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103 antigen; CD103; HML-1 antigen; HUMINAE; Integrin alpha E; integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alphapolypeptide); ITGAE; MGC141996; Mucosal lymphocyte 1 antigen