Mouse JAM-4/IGSF5 Biotinylated Antibody

Catalog #: BAF3836 Datasheet / COA / SDS

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BAF3836

All JAM-4/IGSF5 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse JAM-4/IGSF5 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse JAM‑4/IGSF5 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) JAM-A, rmJAM‑B, and rmJAM-C is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse JAM‑4/IGSF5
Leu15-Ala139
Accession # NP_082354

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse JAM‑4/IGSF5

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-4/IGSF5

JAM-4, also known as IGSF5, is a 93 kDa member of the CTX (Cortical Thymocyte Marker in Xenopus) subfamily of the Ig superfamily. It is a type I transmembrane protein with an N-terminal extracellular V-type and C2 type Ig domain separated by a J segment, a transmembrane domain and a cytoplasmic tail with a PDZ binding motif. JAM-4 is expressed in intestinal, renal, and mammary epithelial cells, spermatogonia, and hematopoietic stem cells. It is a cell adhesion molecule that mediates homophilic interaction. JAM-4 also interacts with the intracellular membrane-associated guanylate kinase inverted 1 (MAGI-1). An additional isoform containing a 100 residue insertion between Gln 125 and Asp 126 has been reported. Over the region used as immunogen, mouse JAM-4 shares 82% and 63% amino acid sequence identity with rat and human JAM-4, respectively.

Long Name

Junctional Adhesion Molecule 4

Entrez Gene IDs

150084 (Human); 72058 (Mouse)

Alternate Names

GSF5; IGSF5; Immunoglobulin Superfamily 5 Like; Immunoglobulin Superfamily Member 5; Immunoglobulin Superfamily, Member 5; JAM4; JAM-4; JCAM; Junctional Adhesion Molecule 4; Putative Gene, Immunoglobulin Superfamily 5 Like11