Mouse Kell Antibody

Catalog #: MAB1454 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1454
MAB1454-SP

All Kell Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Kell Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Kell in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ECE-2, rhNeprilysin, or recombinant mouse Neprilysin is observed.

Source

Monoclonal Rat IgG_2A Clone # 214711

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Kell
Phe50-Trp713
Accession # Q9EQF2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Kell (Catalog # 1454-ZN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kell

Kell, a type II membrane glycoprotein, is linked through a single disulfide bond to XK, a putative membrane transporter. The two proteins constitute the Kell blood group antigens (1). Kell is a zinc endopeptidase of the neprilysin (NEP) family which also includes endothelin converting enzymes (ECE-1 and ECE-2), PEX, XCE, DINE and several NEP-like proteins (2). It has been shown to cleave big endothelin-3 (ET-3) at Trp21-Ile22, yielding ET-3, and to a much lesser extent, big ET-1 and big ET-2 at Trp21-Val22, yielding ET-1 and ET-2 (3). Several different molecular defects cause the Kell null phenotype, which has no obvious clinical outcome (4).

References

Lee, S. et al. (2000) Transfus. Med. Rev. 14:93.
Turner, A.J. et al. (2001) BioEssays. 23:261.
Lee, S. et al. (1999) Blood 94:1440.
Lee, S. et al. (2001) J. Biol. Chem. 276:27281.

Long Name

Zinc protease blood group antigen

Entrez Gene IDs

3792 (Human); 23925 (Mouse)

Alternate Names

CD238 antigen; CD238; EC 3.4.24.-; ECE3; KEL; kell blood group antigen; kell blood group glycoprotein; Kell blood group; Kell blood group, metalloendopeptidase; Kell blood group, metallo-endopeptidase; Kell