Mouse Klotho Biotinylated Antibody

Catalog #: BAF1819
4 Citations Datasheet / COA / SDS

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BAF1819

All Klotho Antibodies

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Citations (4)

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Mouse Klotho Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Klotho in Western blots. In this format, approximately 10% cross-reactivity with recombinant mouse beta Klotho is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Klotho
Arg31-His550
Accession # BAA25307

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Klotho (Catalog # 1819-KL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Klotho

Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1014 amino acid (aa) type I transmembrane protein contains a 34 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, mouse Klotho shares 95%, 87% and 87% aa identity with rat, human and equine Klotho, respectively. Although a truncated 554 aa isoform predicts a soluble 70 kDa form, the soluble form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)₂D₃ (vitamin D) (7). Klotho-deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10 - 12).

References

Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11.
Kuro-o, M. et al. (1997) Nature 390:45.
Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6.
Imura, A. et al. (2004) FEBS Lett. 565:143.
Kurosu, H. et al. (2005) Science 309:1829.
Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
Chang, Q. et al. (2005) Science 310:490.
Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.
Liu, H. et al. (2007) Science 317:803.
Imura, A. et al. (2007) Science 316:1615.

Entrez Gene IDs

9365 (Human); 16591 (Mouse)

Alternate Names

EC 3.2.1; EC 3.2.1.31; KL; Klotho

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Citations for Mouse Klotho Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Multiplexing Biosensor for the Detection of Extracellular Vesicles as Biomarkers of Tissue Damage and Recovery after Ischemic Stroke
Authors: Picciolini, S;Mangolini, V;Rod�, F;Montesano, A;Arnaboldi, F;Liuzzi, P;Mannini, A;Bedoni, M;Gualerzi, A;
International journal of molecular sciences
Species: Human
Sample Types: Extracellular Vesicles
Applications: Surface Plasmon Resonance (SPR)
The biphasic and age-dependent impact of klotho on hallmarks of aging and skeletal muscle function
Authors: Zachary Clemens, Sruthi Sivakumar, Abish Pius, Amrita Sahu, Sunita Shinde, Hikaru Mamiya et al.
eLife
The Klotho proteins in health and disease
Authors: M Kuro-O
Nat Rev Nephrol, 2019-01-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Circulating alphaKlotho influences phosphate handling by controlling FGF23 production.
Authors: Smith R
J. Clin. Invest., 2012-11-26;122(12):4710-5.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development