Mouse KLRG1 Antibody

Catalog #: MAB69441
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB69441-100
MAB69441-SP

Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry.

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All KLRG1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse KLRG1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse KLRG1 in direct ELISAs.

Source

Monoclonal Hamster IgG Clone # 2F1

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

IL-2 activated NK cells from C57/BL6 mice
Accession # O88713

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of KLRG1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat anti-Mouse CD161/NK1.1 PE-conjugated Monoclonal Antibody (Catalog # FAB8319P) and either (A) Hamster Anti-Mouse KLRG1 Monoclonal Antibody (Catalog # MAB69441) or (B) Hamster IgG control antibody followed by APC-conjugated Anti-Hamster IgG Secondary Antibody. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLRG1

KLRG1 (Killer cell Lectin‑like Receptor G1), also called MAFA (Mast cell Function Associated), is an inhibitory type II transmembrane glycoprotein of the C‑type lectin family, designated CLEC15A (1). Mature mouse KLRG1 consists of a 33 amino acid (aa) cytoplasmic domain with one Immunoreceptor Tyrosine‑based Inhibitory Motif (ITIM), a 23 aa transmembrane segment, and a 132 aa extracellular domain (ECD) with one C‑type lectin domain (CTLD) (2). Within the ECD, mouse KLRG1 shares 57% and 80% aa sequence identity with human and rat KLRG1, respectively. Alternate splicing generates additional isoforms of mouse KLRG1 that lack either the CTLD or the CTLD, transmembrane segment, and a portion of the cytoplasmic domain (3). KLRG1 is expressed as a 30 ‑ 40 kDa N‑glycosylated molecule that forms disulfide‑linked homodimers, trimers, and tetramers (4, 5). It is expressed on subpopulations of CD8⁺, CD4⁺, regulatory, and gamma/delta T cells as well as on NK cells (2, 4, 6 ‑ 8). KLRG1 is expressed on T cells found in cord blood, but it is down‑regulated postnatally and is subsequently re‑expressed on antigen‑exposed T cells (7, 9). It is expressed by a greater proportion of CD8⁺ T cells in the elderly and by virus‑specific CD8⁺ T cells during chronic virus infection (10 ‑ 12). KLRG1 binds to E-, N-, and R-Cadherins, triggering ITIM‑dependent KLRG1 signaling and inhibition of T cell activation (5, 13, 14). The response is bi‑directional, as KLRG1 binding to E‑Cadherin on dendritic cells (DC) can induce an anti‑inflammatory DC phenotype (increased IL‑10 production and decreased IL‑6 and TNF‑ alpha production) (15).

References

Henson, S.M. and A.N. Akbar (2009) Age 31:285.
Hanke, T. et al. (1998) Eur. J. Immunol. 28:4409.
Voehringer, D. et al. (2001) Immunogenetics 52:206.
Corral, L. et al. (2000) Eur. J. Immunol. 30:920.
Rosshart, S. et al. (2008) Eur. J. Immunol. 38:3354.
Voehringer, D. et al. (2002) Blood 100:3698.
Beyersdorf, N. et al. (2007) Eur. J. Immunol. 37:3445.
Eberl, M. et al. (2005) J. Leukoc. Biol. 77:67.
Marcolino, I. et al. (2004) Eur. J. Immunol. 34:2672.
Ouyang, Q. et al. (2003) Exp. Gerontol. 38:911.
Thimme, R. et al. (2005) J. Virol. 79:12112.
Cush, S.S. and E. Flano (2011) J. Immunol. 186:4051.
Ito, M. et al. (2006) J. Exp. Med. 203:289.
Tessmer, M.S. et al. (2007) Int. Immunol. 19:391.
Banh, C. et al. (2009) Blood 114:5299.

Long Name

Killer Cell Lectin-like Receptor Subfamily G Member 1

Entrez Gene IDs

10219 (Human); 50928 (Mouse); 58975 (Rat)

Alternate Names

2F1; CLEC15A; CLEC15AMAFA-LIKE; C-type lectin domain family 15 member A; C-type lectin domain family 15, member A; ITIM-containing receptor MAFA-L; killer cell lectin-like receptor subfamily G member 1; killer cell lectin-like receptor subfamily G, member 1; KLRG1; MAFA; MAFAL; MAFA-L; MAFA-like receptor; MAFAMAFA-2F1; mast cell function-associated antigen (ITIM-containing); Mast cell function-associated antigen; MGC13600

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Citation for Mouse KLRG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Functionalized nanowires for miRNA-mediated therapeutic programming of naïve T cells
Authors: Yee Mon, KJ;Kim, S;Dai, Z;West, JD;Zhu, H;Jain, R;Grimson, A;Rudd, BD;Singh, A;
Nature nanotechnology
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry