Mouse Lefty-2 Antibody

Catalog #: MAB7648 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7648
MAB7648-SP

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Mouse Lefty-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Lefty-2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Lefty-A or recombinant mouse Lefty-1 is observed.

Source

Monoclonal Rat IgG_2B Clone # 836101

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Lefty-2
Phe78-Leu368
Accession # P57785

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Lefty-2 in D3 Mouse Stem Cell Line. Lefty-2 was detected in immersion fixed D3 mouse embryonic stem cell line cultured with (upper panel) or without (lower panel) Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) using Rat Anti-Mouse Lefty-2 Monoclonal Antibody (Catalog # MAB7648) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the perinuclear region. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lefty-2

Lefty-2 (Left-right determination factor 2; also Lefty-B [in human]) is a atypical member of the TGF-beta family of proteins. It is expressed during early embryogenesis in both the primitive streak and left-side lateral plate mesoderm. In the adult, Lefty-2 appears in oviduct epithelium. Lefty-2 acts in a manner reminiscent of that for Chordin and Noggin, and it is assumed that Lefty-2 is an antagonist of BMP activity. Notably, Lefty-2 and Nodal are likely under the control of Lefty-1, and thus all three molecules would appear to contribute to the creation of a left side-type body plan. Mouse Lefty-2 is synthesized as a 368 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, plus a 347 aa, 41-42 kDa bioactive proprecursor that may undergo proteolytic processing at one of two downstream cleavage sites. If cleavage occurs after Arg77, the resulting 33-34 kDa mature form (aa 78-368) is biologically inactive; if cleavage occurs after Arg135, the resulting 27-28 kDa mature form (aa 136-368) is biologically active. Lefty-2 is not a covalent homodimer and has been suggested to act as a monomer. Over aa 78-368, mouse Lefty-2 shares 94%, 83% and 95% aa sequence identity with rat Lefty-2, human Lefty-B and mouse Lefty-1, respectively.

Entrez Gene IDs

7044 (Human); 320202 (Mouse); 289316 (Rat)

Alternate Names

Ebaf; Endometrial Bleeding-Associated Factor; LEFTA; Left-Right Determination Factor 2; Left-right determination factor A; Lefty-2; LEFTYA; Protein Lefty-2; Protein Lefty-A; TGFB4; TGF-beta 4; TGF-beta-4; Transforming Growth Factor Beta-4