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Mouse Leptin R Biotinylated Antibody

Catalog #: BAF497
38 Citations Datasheet / COA / SDS
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Detection of Leptin R in HEK293 Human Cell Line transfected with Mouse Leptin R and EGFP by Flow Cytometry.
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Citations (38)
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Mouse Leptin R Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Leptin R in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
Ala20-Gly839
Accession # Q3US58
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR)
Flow Cytometry
0.25 µg/106 cells
HEK293 human cell line transfected with mouse Leptin R and EGFP

Mouse Leptin R Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Leptin R Antibody (Catalog # MAB497)

Standard: Recombinant Mouse Leptin R Fc Chimera Protein (Catalog # 497-LR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Leptin R in HEK293 Human Cell Line transfected with Mouse Leptin R and EGFP by Flow Cytometry. HEK293 human cell line transfected with Mouse Leptin R and EGFP was stained with (A) Goat Anti-Mouse Leptin R Biotinylated Polyclonal Antibody (Catalog # BAF497) or (B) control antibody (BAF108), followed by Allophycocyanin-conjugated Streptavidin (F0050). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

References
  1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  3. Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
  4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
  5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.
Long Name
Leptin Receptor
Entrez Gene IDs
3953 (Human); 16847 (Mouse)
Alternate Names
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295

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Citations for Mouse Leptin R Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Induction of a Timed Metabolic Collapse to Overcome Cancer Chemoresistance
    Authors: Van Gastel N, Spinelli Jb, Sharda A Et Al.
    Cell Metab.
  2. Oncolytic Viruses Engineered to Enforce Leptin Expression Reprogram Tumor-Infiltrating T Cell Metabolism and Promote Tumor Clearance
    Authors: Dayana B. Rivadeneira, Kristin DePeaux, Yiyang Wang, Aditi Kulkarni, Tracy Tabib, Ashley V. Menk et al.
    Immunity
  3. Pharmacological Inhibition of Inositol Hexakisphosphate Kinase 1 Protects Mice against Obesity-Induced Bone Loss
    Authors: Siddaraju V. Boregowda, Manjunatha K. Nanjappa, Cori N. Booker, Jacqueline Strivelli, Valentina M. Supper, Paul S. Cooke et al.
    Biology (Basel)
  4. Ruxolitinib improves hematopoietic regeneration by restoring mesenchymal stromal cell function in acute graft-versus-host disease
    Authors: Yan Lin, Quan Gu, Shihong Lu, Zengkai Pan, Zining Yang, Yapu Li et al.
    Journal of Clinical Investigation
  5. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping
    Authors: N Severe, NM Karabacak, K Gustafsson, N Baryawno, G Courties, Y Kfoury, KD Kokkaliari, C Rhee, D Lee, EW Scadden, JE Garcia-Rob, T Brouse, M Nahrendorf, M Toner, DT Scadden
    Cell Stem Cell, 2019-07-03;0(0):.
  6. Distinct skeletal stem cell types orchestrate long bone skeletogenesis
    Authors: Ambrosi TH, Sinha R, Steininger HM et al.
    eLife
  7. Fibrinolytic factor-mediated crosstalk with endothelial cells expands murine bone marrow mesenchymal stromal cells
    Authors: Douaa Dhahri
    Blood, 2016-06-09;0(0):.
  8. Mesenchymal loss of p53 alters stem cell capacity and models human soft tissue sarcoma traits
    Authors: Yuriko Sorimachi, Hiroshi Kobayashi, Yusuke Shiozawa, Shuhei Koide, Ryuichiro Nakato, Yukiko Shimizu et al.
    Stem Cell Reports
  9. Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1.
    Authors: Ke, X, Do, D C Et al.
    J Allergy Clin Immunol
  10. Enzyme‐Cleaved Bone Marrow Transplantation Improves the Engraftment of Bone Marrow Mesenchymal Stem Cells
    Authors: Hotaka Kawai, May Wathone Oo, Kiyofumi Takabatake, Ikue Tosa, Yamin Soe, Htoo Shwe Eain et al.
    JBMR Plus
  11. Bone marrow stromal cell-derived hepcidin has antimicrobial and immunomodulatory activities
    Authors: Krepuska, M;Mayer, B;Vitale-Cross, L;Myneni, VD;Boyajian, MK;Németh, K;Szalayova, I;Cho, T;Mc Clain-Caldwell, I;Gingerich, AD;Han, H;Westerman, M;Rada, B;Mezey, É;
    Scientific reports
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  12. Restoring bone marrow niche function rejuvenates aged hematopoietic stem cells by reactivating the DNA Damage Response
    Authors: P Ramalingam, MC Gutkin, MG Poulos, T Tillery, C Doughty, A Winiarski, AG Freire, S Rafii, D Redmond, JM Butler
    Nature Communications, 2023-04-10;14(1):2018.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC, MACS
  13. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
    Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
    Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis
    Authors: MJ Schloss, M Hulsmans, D Rohde, IH Lee, N Severe, BH Foy, FE Pulous, S Zhang, KD Kokkaliari, V Frodermann, G Courties, C Yang, Y Iwamoto, AS Knudsen, CS McAlpine, M Yamazoe, SP Schmidt, GR Wojtkiewic, GS Masson, K Gustafsson, D Capen, D Brown, JM Higgins, DT Scadden, P Libby, FK Swirski, K Naxerova, M Nahrendorf
    Nature Immunology, 2022-03-28;23(4):605-618.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Microbiota-derived lactate promotes hematopoiesis and erythropoiesis by inducing stem cell factor production from leptin receptor+ niche cells
    Authors: YS Lee, TY Kim, Y Kim, S Kim, SH Lee, SU Seo, BO Zhou, O Eunju, KS Kim, MN Kweon
    Experimental & Molecular Medicine, 2021-09-09;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC
  16. A mechanosensitive peri-arteriolar niche for osteogenesis and lymphopoiesis
    Authors: B Shen, A Tasdogan, JM Ubellacker, J Zhang, ED Nosyreva, L Du, MM Murphy, S Hu, Y Yi, N Kara, X Liu, S Guela, Y Jia, V Ramesh, C Embree, EC Mitchell, YC Zhao, LA Ju, Z Hu, GM Crane, Z Zhao, R Syeda, SJ Morrison
    Nature, 2021-02-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Inhibition of longevity regulator PAPP-A modulates tissue homeostasis via restraint of mesenchymal stromal cells
    Authors: M Mohrin, J Liu, J Zavala-Sol, S Bhargava, J Maxwell Tr, A Brito, D Hu, D Brooks, G Koukos, L Alabdulaal, JS Paw, K Hake, G Kolumam, ML Bouxsein, R Baron, Y Kutskova, A Freund
    Aging Cell, 2021-02-09;0(0):e13313.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Leptin receptor, a surface marker for a subset of highly engrafting long-term functional hematopoietic stem cells
    Authors: T Trinh, J Ropa, A Aljoufi, S Cooper, A Sinn, EF Srour, HE Broxmeyer
    Leukemia, 2020-11-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. C-KIT Expression Distinguishes Fetal from Postnatal Skeletal Progenitors
    Authors: DD He, XT Tang, W Dong, G Cui, G Peng, X Yin, Y Chen, N Jing, BO Zhou
    Stem Cell Reports, 2020-03-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Leptin receptor-expressing cells represent a distinct subpopulation of notochord-derived cells and are essential for disc homoeostasis
    Authors: B Gao, J Yin, X Xu, J Fan, D Wang, C Zheng, W Lu, P Cheng, J Sun, D Wang, L Li, BO Zhou, L Yang, Z Luo
    J Orthop Translat, 2019-12-24;21(0):91-99.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Congenital lipodystrophy induces severe osteosclerosis
    Authors: W Zou, N Rohatgi, JR Brestoff, Y Zhang, EL Scheller, CS Craft, MD Brodt, N Migotsky, MJ Silva, CA Harris, SL Teitelbaum
    PLoS Genet., 2019-06-24;15(6):e1008244.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Nicotinamide mononucleotide promotes osteogenesis and reduces adipogenesis by regulating mesenchymal stromal cells via the SIRT1 pathway in aged bone marrow
    Authors: J Song, J Li, F Yang, G Ning, L Zhen, L Wu, Y Zheng, Q Zhang, D Lin, C Xie, L Peng
    Cell Death Dis, 2019-04-18;10(5):336.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Macrophage-lineage TRAP+ cells recruit periosteum-derived cells for periosteal osteogenesis and regeneration
    Authors: B Gao, R Deng, Y Chai, H Chen, B Hu, X Wang, S Zhu, Y Cao, S Ni, M Wan, L Yang, Z Luo, X Cao
    J. Clin. Invest., 2019-04-04;129(6):2578-2594.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: Flow Cytometry, IHC
  24. Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche
    Authors: M Balzano, M De Grandis, TP Vu Manh, L Chasson, F Bardin, A Farina, A Sergé, G Bidaut, P Charbord, L Hérault, AL Bailly, A Cartier-Mi, A Boned, M Dalod, E Duprez, P Genever, M Coles, M Bajenoff, L Xerri, M Aurrand-Li, C Schiff, SJC Mancini
    Cell Rep, 2019-03-19;26(12):3257-3271.e8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells
    Authors: M Zhao, F Tao, A Venkatrama, Z Li, SE Smith, J Unruh, S Chen, C Ward, P Qian, JM Perry, H Marshall, J Wang, XC He, L Li
    Cell Rep, 2019-01-15;26(3):652-669.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Circulating healing (CH) cells expressing BST2 are functionally activated by the injury-regulated systemic factor HGFA
    Authors: C Lo Sicco, D Reverberi, F Villa, U Pfeffer, R Quarto, R Cancedda, R Tasso
    Stem Cell Res Ther, 2018-11-08;9(1):300.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice
    Authors: B Gao, X Lin, H Jing, J Fan, C Ji, Q Jie, C Zheng, D Wang, X Xu, Y Hu, W Lu, Z Luo, L Yang
    Aging Cell, 2018-02-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016-12-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Identification of Senescent Cells in the Bone Microenvironment
    Authors: Joshua N Farr
    J Bone Miner Res, 2016-10-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection
  30. High efficiency cell-specific targeting of cytokine activity.
    Authors: Garcin G, Paul F, Staufenbiel M, Bordat Y, Van der Heyden J, Wilmes S, Cartron G, Apparailly F, De Koker S, Piehler J, Tavernier J, Uze G
    Nat Commun, 2014-01-01;5(0):3016.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Leptin protects host cells from Entamoeba histolytica cytotoxicity by a STAT3-dependent mechanism.
    Authors: Marie CS, Verkerke HP, Paul SN
    Infect. Immun., 2012-02-13;80(5):1934-43.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  32. Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution.
    Authors: Gruver AL, Ventevogel MS, Sempowski GD
    J. Endocrinol., 2009-07-08;203(1):75-85.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  33. Hematopoietic Cell Autonomous Disruption of Hematopoiesis in a Germline Loss-of-function Mouse Model of RUNX1-FPD
    Authors: Martijn P. T. Ernst, Eline Pronk, Claire van Dijk, Paulina M. H. van Strien, Tim V. D. van Tienhoven, Michiel J. W. Wevers et al.
    HemaSphere
  34. Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton
    Authors: Rui Yue, Bo Shen, Sean J Morrison
    eLife
  35. Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments
    Authors: Shuji Nosho, Ikue Tosa, Mitsuaki Ono, Emilio Satoshi Hara, Kei Ishibashi, Akihiro Mikai et al.
    International Journal of Molecular Sciences
  36. Long noncoding RNA Bmncr regulates mesenchymal stem cell fate during skeletal aging.
    Authors: Li CJ, Xiao Y, Yang M et al.
    J. Clin. Invest.
  37. A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node
    Authors: Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, Mina Kozai, Izumi Ohigashi, Yoichi Kurosawa et al.
    Frontiers in Immunology
  38. Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageing
    Authors: Carl A. Mitchell, Evgenia V. Verovskaya, Fernando J. Calero-Nieto, Oakley C. Olson, James W. Swann, Xiaonan Wang et al.
    Nature Cell Biology

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