Mouse LIF Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody. Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA). The ND50 is typically 80-480 ng/mL.
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LIF in Mouse Trigeminal Ganglion. LIF was detected in perfusion fixed frozen sections of mouse trigeminal ganglion using Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to sensory neurons in trigeminal ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIF
Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.
Product Datasheets
Citations for Mouse LIF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice
Authors: Wei Cai, Ligen Shi, Jingyan Zhao, Fei Xu, Connor Dufort, Qing Ye et al.
Journal of Clinical Investigation
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The astrocytic TRPA1 channel mediates an intrinsic protective response to vascular cognitive impairment via LIF production
Authors: Kakae, M;Nakajima, H;Tobori, S;Kawashita, A;Miyanohara, J;Morishima, M;Nagayasu, K;Nakagawa, T;Shigetomi, E;Koizumi, S;Mori, Y;Kaneko, S;Shirakawa, H;
Science advances
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells, In Vivo
Applications: In vivo assay, Neutralization -
Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis
Authors: S Xu, X Yang, Q Chen, Z Liu, Y Chen, X Yao, A Xiao, J Tian, L Xie, M Zhou, Z Hu, F Zhu, X Xu, F Hou, J Nie
EBioMedicine, 2022-11-04;86(0):104312.
Species: Mouse
Sample Types: In Vivo, Whole Tissue
Applications: IHC, In Vivo -
KAI1(CD82) is a key molecule to control angiogenesis and switch angiogenic milieu to quiescent state
Authors: JW Lee, J Hur, YW Kwon, CW Chae, JI Choi, I Hwang, JY Yun, JA Kang, YE Choi, YH Kim, SE Lee, C Lee, DH Jo, H Seok, BS Cho, SH Baek, HS Kim
Journal of hematology & oncology, 2021-09-16;14(1):148.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Sclerostin expression in trabecular bone is downregulated by osteoclasts
Authors: M Koide, T Yamashita, K Murakami, S Uehara, K Nakamura, M Nakamura, M Matsushita, T Ara, H Yasuda, JM Penninger, N Takahashi, N Udagawa, Y Kobayashi
Sci Rep, 2020-08-13;10(1):13751.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Mapping the Effects of Genetic Variation on Chromatin State and Gene Expression Reveals Loci That Control Ground State Pluripotency
Authors: DA Skelly, A Czechanski, C Byers, S Aydin, C Spruce, C Olivier, K Choi, DM Gatti, N Raghupathy, GR Keele, A Stanton, M Vincent, S Dion, I Greenstein, M Pankratz, DK Porter, W Martin, C O'Connor, W Qin, AH Harrill, T Choi, GA Churchill, SC Munger, CL Baker, LG Reinholdt
Cell Stem Cell, 2020-08-13;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization, Reference Standard -
Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy
Authors: SS Welc, I Flores, M Wehling-He, J Ramos, Y Wang, C Bertoni, JG Tidball
Nat Commun, 2019-06-26;10(1):2788.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cachexia-associated adipose loss induced by tumor-secreted leukemia inhibitory factor is counterbalanced by decreased leptin
Authors: GK Arora, A Gupta, S Narayanan, T Guo, P Iyengar, RE Infante
JCI Insight, 2018-07-26;3(14):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate
Authors: Emma L Rawlins
Development, 2016-08-30;143(20):3686-3699.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Heart failure causes cholinergic transdifferentiation of cardiac sympathetic nerves via gp130-signaling cytokines in rodents.
Authors: Kanazawa H, Ieda M, Kimura K, Arai T, Kawaguchi-Manabe H, Matsuhashi T, Endo J, Sano M, Kawakami T, Kimura T, Monkawa T, Hayashi M, Iwanami A, Okano H, Okada Y, Ishibashi-Ueda H, Ogawa S, Fukuda K
J. Clin. Invest., 2010-01-04;120(2):408-21.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Osteoblast and Osteoclast Activity Affect Bone Remodeling Upon Regulation by Mechanical Loading-Induced Leukemia Inhibitory Factor Expression in Osteocytes
Authors: Jingke Du, Jiancheng Yang, Zihao He, Junqi Cui, Yiqi Yang, Mingming Xu et al.
Frontiers in Molecular Biosciences
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Dihydroartemisinin attenuates osteoarthritis by inhibiting abnormal bone remodeling and angiogenesis in subchondral bone
Authors: Long Ma, Xin Zhao, Yibin Liu, Jiang Wu, Xiaochun Yang, Qunhua Jin
International Journal of Molecular Medicine
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