Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 673602
View all LIFR alpha Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse LIF R alpha
Leu44-Ser828
Accession # P42703

Specificity

Detects mouse LIF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LIF R alpha is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse LIFR alpha Antibody

Detection of LIF Ra antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of LIF R alpha in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse LIF Ra Monoclonal Antibody (Catalog # MAB5990, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Applications for Mouse LIFR alpha Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample:
D3 mouse embryonic stem cell line

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha ), also known as LIFR beta and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain (2, 3). Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD (4). LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity (5). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (6, 7). The LIF R alpha /gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (6). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (8, 9). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (10, 11). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (4, 12-14).

References

  1. Muller-Newen, G. (2003) Science STKE 2003:pe40.
  2. Gearing, D.P. et al. (1991) EMBO J. 10:2839.
  3. Tomida, M. et al. (1994) J. Biochem. 115:557.
  4. Owczarek, C.M. et al. (1996) J. Biol. Chem. 271:5495.
  5. Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
  6. Gearing, D.P. et al. (1992) Science 255:1434.
  7. Giese, B. et al. (2005) J. Cell Sci. 118:5129.
  8. Ip, N.Y. et al. (1992) Cell 69:1121.
  9. Davis, S. et al. (1993) Science 260:1805.
  10. Metcalf, D (2003) Stem Cells 21:5.
  11. Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
  12. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  13. Stewart, C.L. et al. (1992) Nature 359:76.
  14. Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.

Long Name

Leukemia Inhibitory Factor Receptor alpha

Alternate Names

CD118, LIF R alpha, LIF R beta, LIFR, LIFRa

Entrez Gene IDs

3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)

Gene Symbol

LIFR

UniProt

P42703

Additional LIFR alpha Products

Product Documents for Mouse LIFR alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse LIFR alpha Antibody

For research use only

Citations for Mouse LIFR alpha Antibody

Customer Reviews for Mouse LIFR alpha Antibody

There are currently no reviews for this product. Be the first to review Mouse LIFR alpha Antibody and earn rewards!

Have you used Mouse LIFR alpha Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies