Mouse Lipin 3 Antibody

Catalog #: AF4064 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4064
AF4064-SP

All Lipin 3 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Lipin 3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Lipin 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human (rh) Lipin 3 is observed and less than 5% cross-reactivity with rhLipin 1 and rhLipin 2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Lipin 3
Thr428-Arg529
Accession # Q99PI4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Lipin 3

Immunocytochemistry

5-15 µg/mL

Immersion fixed CCD-18co human colon fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipin 3

Mouse Lipin 3 is a 94 kDa member of the small Lipin family of nuclear proteins. It is primarily expressed in the GI tract and may play a role in DAG production or membrane synthesis. Lipin 3 is 848 amino acids (aa) in length. It shows conserved N- and C-terminal domains that flank an NLS between aa 135‑144. Lipin 3 has modest phosphatidate phosphatase activity. Two potential isoforms exist; one shows a deletion of aa 507‑537, while the other shows a 10 aa insertion between Glu 506 and Ser 507. Over aa 428‑549, mouse Lipin 3 shares 84% and 96% aa sequence identity with human and rat Lipin 3, respectively.

Long Name

Phosphatidate Phosphatase LPIN3

Entrez Gene IDs

64900 (Human); 64899 (Mouse); 362261 (Rat)

Alternate Names

dJ450M14.2; dJ450M14.3; dJ620E11.2; EC 3.1.3.4; Lipin 3; lipin 3-like; lipin-3; Lipin-3-like; LIPN3L; LPIN3; phosphatidate phosphatase LPIN3; SMP2