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Mouse LRP-6 Antibody

Catalog #: MAB2960
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2960
MAB2960-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse LRP-6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LRP-6 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 50 - 60% cross-reactivity with recombinant human (rh) LRP-6 and no cross-reactivity with rhLDL R.
Source
Monoclonal Rat IgG2B Clone # 290913
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse LRP-6
Met1-Pro1368
Accession # O88572
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse LRP‑6 (Catalog # 2960-LR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-6

The low-density lipoprotein (LDL) receptor-related protein 5 (LRP-5) and LRP-6 constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins (FZD) (2, 3, 4). The mouse LRP-6 cDNA encodes a 1613 amino acid (aa) residue precursor including a 19 aa signal sequence, 1351 aa extracellular domain (ECD), a 23 aa transmembrane (TM) segment, and a 20 aa cytoplasmic domain (5). The ECD contains 20 YWTD motif-containing LDLR-B domains, fourEGF-like repeats, and three cysteine-rich LDLR-A repeats. The ECD of mouse LRP-6 shares 71% aa sequence identity with the ECD of mouse LRP-5 and 98% aa sequence identity with the ECD of human, rat, and canine LRP-6. The intracellular region of LRP-6 contains repeated PPPSP motifs. When the Ser/Thr in these motifs are phosphorylated, LRP-6 can interact with Axin and propagate canonical Wnt signal transduction (6, 7). LRP-6 forms inactive homodimers via its YWTD-EGF domains (8). Wnt binding to FZD and subsequent association with LRP-6 lead to activating conformational changes in LRP-6 cytoplasmic domains (8). LRP-6 can also interact directly with the Dickkopf (Dkk), sclerostin, and Wise proteins which are modulators of Wnt signaling (9 - 14). Formation of a ternary complex of LRP-6, Dkk-1, and Kremen triggers the internalization of the complex and removal LRP-6 from the cell surface. LRP-5 and LRP-6 share overlapping functions in diverse embryonic developmental processes (15).

References
  1. Schneider, W.J. and J. Nimpf (2003) Cell Mol Life Sci. 60:892.
  2. He, X. et al. (2004) Development 131:1663.
  3. Seto, E.S. and H.J. Bellen (2004) Trends Cell Biol. 14:45. 
  4. Pinson, K.I. et al. (2000) Nature 407:535. 
  5. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
  6. Tamai, K. et al. (2000) Nature 407:530. 
  7. Tamai, K. et al. (2004) Mol. Cell 13:149. 
  8. Liu, G. et al. (2003) Mol. Cell. Biol. 23:5825.
  9. Mao, B. et al. (2001) Nature 411:321.
  10. Semenov, M.V. et al. (2001) Curr. Biol. 11:951.
  11. Bafico, A. et al. (2001) Nat. Cell Biol. 3:683.
  12. Li, X. et al. (2005) J. Biol. Chem. 280:19883.
  13. Semenov, M. et al. (2005) J. Biol. Chem. 280:26770.
  14. Itasaki, N. et al. (2003) Development 130:4295.
  15. Kelly, O. et al. (2004) Development 131:2803.
Long Name
LDL Receptor-related Protein 6
Entrez Gene IDs
4040 (Human); 16974 (Mouse)
Alternate Names
ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6

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Citations for Mouse LRP-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mutant APC reshapes Wnt signaling plasma membrane nanodomains by altering cholesterol levels via oncogenic beta -catenin
    Authors: Alfredo Erazo-Oliveras, Mónica Muñoz-Vega, Mohamed Mlih, Venkataramana Thiriveedi, Michael L. Salinas, Jaileen M. Rivera-Rodríguez et al.
    Nature Communications
  2. Cripto-1 enhances the canonical Wnt/β-catenin signaling pathway by binding to LRP5 and LRP6 co-receptors.
    Cell Signal, 2012-09-27;25(1):178-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC

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