Mouse Lymphotoxin beta R/TNFRSF3 Antibody

Catalog #: MAB10081
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10081
MAB10081-SP

Detection of Lymphotoxin beta R/TNFRSF3 in NIH‑3T3 Mouse Cell Line by Flow Cytometry.

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All Lymphotoxin beta R/TNFRSF3 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Lymphotoxin beta R/TNFRSF3 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Lymphotoxin beta R in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) 4‑1BB, rmBAFF R, rmCD27, rmCD30, rmCD40, recombinant human (rh) DR3, rhDR6, rmEDAR, rmFas, rmGITR, rmHVEM, rhLymphotoxin beta R, rmNGF R, rmOPG, rmOX40, rmRANK, rhRELT, rmTAJ, rmTNF RI, rmTNF RII, rhTRADD, rmTRAIL R2, rhTRAIL R3, rhTRAIL R4, or rmTWEAK R.

Source

Monoclonal Rat IgG_2A Clone # 157108

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Lymphotoxin beta R/TNFRSF3
Ser28-Pro218
Accession # P50284

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Lymphotoxin beta R/TNFRSF3
under non-reducing conditions only

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Lymphotoxin beta R/TNFRSF3 in NIH‑3T3 Mouse Cell Line by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line was stained with Rat Anti-Mouse Lymphotoxin beta R/TNFRSF3 Monoclonal Antibody (Catalog # MAB10081, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta R, now designated TNFRSF3, is a cell surface protein expressed in a variety of tissues. Lymphotoxin beta R transduces signals following binding of LIGHT or the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. It plays a critical role in controlling cellular immune functions and lymphoid organogenesis.

Long Name

Lymphotoxin beta Receptor

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Alternate Names

CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse Lymphotoxin beta R/TNFRSF3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Conditional Deletion of NF-kappaB-Inducing Kinase (NIK) in Adult Mice Disrupts Mature B Cell Survival and Activation.
Authors: Brightbill H, Jackman J, Suto E, Kennedy H, Jones C, Chalasani S, Lin Z, Tam L, Roose-Girma M, Balazs M, Austin C, Lee W, Wu L
J Immunol, 2015-06-26;195(3):953-64.
Species: Mouse
Sample Types: Cell Lysates
Applications: Bioassay