Mouse M-Cadherin/Cadherin-15 Antibody

Catalog #: MAB7677 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7677
MAB7677-SP

M‑Cadherin/Cadherin‑15 in C2C12 Mouse Cell Line.

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Mouse M-Cadherin/Cadherin-15 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse M-Cadherin/Cadherin-15 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human M-Cadherin, recombinant mouse (rm) Cadherin-13, or rmP-Cadherin is observed.

Source

Monoclonal Rat IgG_2A Clone # 800516

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse M-Cadherin/Cadherin-15
Val22-Ala605
Accession # P33146

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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M‑Cadherin/Cadherin‑15 in C2C12 Mouse Cell Line. M-Cadherin/Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cells differentiated for 5 days with 5% equine serum using Rat Anti-Mouse M-Cadherin/Cadherin-15 Monoclonal Antibody (Catalog # MAB7677) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of developing myotubes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: M-Cadherin/Cadherin-15

CDH-15 (Cadherin 15; also M-cadherin, Muscle cadherin and Cadherin-14) is a 125-127 kDa atypical member of the classical cadherin family, cadherin superfamily of molecules. It is expressed by muscle satellite cells, cells of the embryonic myotome, and hematopoietic bone marrow stem cells. CDH-15 appears to bind homotypically in trans, thus allowing for the identification and subsequent fusion of myoblast precursors, particularly those in slow-twitch (or red fiber) muscles. This is accompanied by a downregulation of mitochondrial induced apoptosis. Mouse CDH-15 is synthesized as a 784 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, a 38 aa propeptide, and a 725 aa mature region. The mature region is expressed as a type I transmembrane glycoprotein that possesses a 546 aa extracellular region (aa 60-605) and a 159 aa cytoplasmic domain (aa 626-784). The extracellular region shows five consecutive cadherin domains. Over aa 22-605, mouse CDH-15 shares 88% and 97% aa sequence identity with human and rat CDH-15, respectively.

Long Name

M-Cadherin [Myotubule]

Entrez Gene IDs

1013 (Human); 12555 (Mouse); 361432 (Rat)

Alternate Names

cadherin 15, M-cadherin (myotubule); cadherin 15, type 1, M-cadherin (myotubule); Cadherin-14; Cadherin-15; cadherin-3; CDH14; CDH14cadherin-14; CDH15; CDH3cadherin-15; CDHM; MCAD; MCadherin; M-Cadherin; MRD3; Muscle cadherin; muscle-cadherin