Mouse M-CSF R PE-conjugated Antibody

Catalog #: FAB3818P
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3818P

Detection of M‑CSF R in J774A.1 Mouse Cell Line by Flow Cytometry.

1 Image

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse M-CSF R PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse M-CSF R in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human M-CSF R, recombinant mouse (rm) PDGF R beta, or rmFlt-3 Ligand is observed.

Source

Monoclonal Rat IgG_2B Clone # 460615

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse M-CSF R
Ala20-Ser511
Accession # P09581

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of M‑CSF R in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse M-CSF R PE-conjugated Monoclonal Antibody (Catalog # FAB3818P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Mouse M-CSF receptor cDNA encodes a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 441 aa cytoplasmic domain. The mouse M-CSF R ECD shares > 99% aa identity with rat and 60-63% aa identity with corresponding sequences in human, canine, feline and bovine M-CSF R. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin alpha _v beta ₃ share signaling pathways during osteoclastogenesis, and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

References

deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
Rovida, E. et al. (2001) J. Immunol. 166:1583.
Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
Dai, X. et al. (2002) Blood 99:111.
Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
Li, M. et al. (2004) J. Neurochem. 91:623.
Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

Long Name

Macrophage Colony Stimulating Factor Receptor

Entrez Gene IDs

1436 (Human); 12978 (Mouse)

Alternate Names

CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; M-CSF R; MCSFR; M-CSFR; Proto-oncogene c-Fms

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse M-CSF R PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Tumor-Released Products Promote Bone Marrow-Derived Macrophage Survival and Proliferation
Authors: JM Motta, VM Rumjanek, A Mantovani, M Locati
Biomedicines, 2021-10-04;9(10):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry