Mouse MARCO Antibody

Name: Mouse MARCO Antibody
Brand: R&D Systems
SKU: MAB2956
Rating: 4 (1 reviews)

Catalog #: MAB2956 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2956-100
MAB2956-SP

Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry

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Mouse MARCO Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MARCO in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 579511

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MARCO
Gln70-Ser518
Accession # Q60754

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MARCO in J774 Mouse Cell Line by Flow Cytometry J774 mouse monocyte-macrophage cell line, resting (open histogram), or treated with 500 ng/ml LPS for 3 days (filled histogram) were stained with Rat Anti-Mouse MARCO Monoclonal Antibody (Catalog # MAB2956) followed by anti-Rat IgG APC-conjugated secondary antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MARCO

Mouse MARCO is a type II transmembrane glycoprotein belonging to the class A scavenger receptor family. It is constitutively expressed in subsets of macrophages found in the marginal zone of the spleen, the peritoneum, and the medullary cord of lymph nodes. The extracellular domains of MARCO form a 220 kDa disulfide-linked homotrimer on the cell surface. MARCO binds both gram positive and gram negative bacteria, as well as oxidized low-density lipoprotein. The amino acid sequence of mouse MARCO extracellular domain is 69% identical to that of human MARCO.

Long Name

Macrophage Receptor with Collagenous Structure/Scavenger Receptor, Class A, Member 2

Entrez Gene IDs

8685 (Human); 17167 (Mouse)

Alternate Names

macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2; MARCO; member 2; SCARA2