Mouse MBL-2 Biotinylated Antibody

Catalog #: BAF2208
2 Citations Datasheet / COA / SDS

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BAF2208

All MBL-2 Antibodies

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Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MBL-2 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MBL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse MBL-1 and recombinant human MBL is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MBL-2
Glu19-Asp244
Accession # P41317

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse MBL‑2 (Catalog # 2208-MB)

Mouse MBL-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse MBL‑2 Antibody (Catalog # AF2208)

Standard: Recombinant Mouse MBL-2 Protein (Catalog # 2208-MB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBL-2

Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which binds to an array of carbohydrate patterns on pathogen surfaces (1, 2). Collectin family members share common structural features: a cysteine rich amino-terminal domain, a collagen-like region, an alpha -helical coiled-coil neck domain and a carboxy terminal C-type (Ca⁺⁺-dependent) lectin or carbohydrate recognition domain (CRD). MBL homotrimerizes to form a structural unit joined byN-terminal disulfide bridges. These homotrimers further associates into oligomeric structures of up to 6 units. Whereas two forms of MBL proteins (MBL-1, also known as S-MBP or MBL-A and MBL-2, also known as L-MBP or MBL-C) exist in rodents and other animals, only one functional MBL protein is present in humans. Mouse MBL-2 shares about 52% and 60% amino acid sequence identity with mouse MBL-1 and human MBL, respectively.

In mouse, MBL-1 and MBL-2 are the only collectins that can activate complement via the lectin complement pathway (1, 2). Serum oligomeric MBL associates with MBL-associated serine protease (MASP) proenzymes. The MBL-MASP proenzyme complex preferentially interact with sugar patterns containing mannose, glucose, L‑fucose, or N-acetyl-glucosamine present at a terminal nonreducing postion on the cell surface of various pathogens and certain tumor cells. This interaction induces pro-enzyme activation and the triggering of the complement cascade, resulting in opsonization and pathogen removal via humoral and cellular immune responses. MBL does not recognize self-components or glycoproteins from other higher animals due to the presence of terminal sialic acid or galactose that interrupts the repeating carbohydrate structures (3). A number of membrane receptors for MBL, including C1q phagocytic receptor (C1qRp), calreticulin (also known as C1qR), and CR1 (CD35), have been described. Interactions with these receptors may also be important in stimulating phagocytosis (1, 2).

Mouse MBL-1 and MBL-2 are produced primarily in the liver and are secreted into the blood stream. In addition, mouse MBL-1 is also expressed in lung, kidney, and testis while MBL-2 is expressed in kidney, thymus, and small intestine (1, 4, 5).

References

Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
Fujita, R. et al. (2004) Immunol. Rev. 198:185.
Saevarsdottir, S. et al. (2004) Scand. J. Immnunol. 60:23.
Uemura, K. et al. (2002) J. Immunol. 169:6945.
Wagner, S. et al. (2003) J. Immunol. 170:1462.

Long Name

Mannose Binding Lectin 2

Entrez Gene IDs

17195 (Mouse)

Alternate Names

L-MBP; mannose-binding protein C; L-MBP; mannose-binding lectin (protein C) 2; MBL; MBL-2; MBL-C; MBP-C

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse MBL-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Role of pentraxin 3 in shaping arthritogenic alphaviral disease: from enhanced viral replication to immunomodulation.
Authors: Foo, Suan-Sin, Chen, Weiqiang, Taylor, Adam, Sheng, Kuo-Chin, Yu, Xing, Teng, Terk-Shi, Reading, Patrick, Blanchard, Helen, Garlanda, Cecilia, Mantovani, Alberto, Ng, Lisa F P, Herrero, Lara J, Mahalingam, Suresh
PLoS Pathog, 2015-02-19;11(2):e1004649.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Binding Assay
Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis.
Authors: Green RS, Stone EL, Tenno M, Lehtonen E, Farquhar MG, Marth JD
Immunity, 2007-08-02;27(2):308-20.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot