Mouse MFG-E8 Antibody Summary
Ala23-Cys463
Accession # P21956
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse MFG‑E8 by Western Blot. Western blot shows lysates of mouse mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MFG-E8 at approximately 60-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse MFG‑E8 by Simple WesternTM. Simple Western lane view shows lysates of recombinant mouse (rm) MFG-E8, loaded at 50 ng/mL. A specific band was detected for MFG-E8 at approximately 87 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse MFG-E8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2805) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human MFG-E8 by Western Blot MFGE8 and NO Are Produced and Secreted by Co-cultured BV2 Microglia Only When Co-cultured in Contact with pFTAA+Neurons(A and B) Representative blot (A) ofMFGE8 in BV2 cells lysates cultured alone or co-cultured in direct contact, or via atranswell, with DRG neurons from 5-month-old P301S-tau, 5-month-old C57, 5-month-old Alz17 mice, or 2-month-old P301S mice.Asterisk: either another isoform ofMFGE8 or a breakdown product. (B) Densitometry of MFGE8 expression normalized to beta -actin; significantly more MFGE8 is produced only in contact co-cultures containing pFTAA+ neurons. Mean± SD, n = 3 independent experiments (*p < 0.05), twoway ANOVA, Dunnett’s correction.(C and D) Elevated MFGE8 (C) or NO (D) in medium conditioned by BV2 cells co-cultured in contact with DRG neurons from5-month-old P301S mice compared with 5-month-old Alz17, 5-month- old C57, or 2-month-old P301S mice (MFGE8: ****p < 0.0001versus all; NO: ****p < 0.0001 versus 5-month-old C57 or 2-month-old P301S mice; ***p < 0.001 versus 5-month-oldAlz17 mice). N.D., none detected. Mean ± SD, n = 3 independent experiments, two-way ANOVA, Bonferroni corrected.(E and F) Elevated MFGE8 (E) or NO (F) in medium conditioned by primary microglia from C57 mice co-cultured in contactwith DRG neurons from 5-month-old P301S mice; microglia from MFGE8 KO mice are negative controls. Mean ± SD, n = 3independent microglial preparations, one-way ANOVA, **p < 0.01 (MFGE8), *p < 0.05 (NO).(G) Increased MFGE8 immunostaining intensity in frontal motor cortex of 5-month-old P301S mice compared with 5-month-oldC57 mice; MFGE8 KO mouse is negative control; 25 μm section at inter- aural 5.12 mm, bregma 1.32 mm; brown, DAB; blue,cresyl violet. Scale bar, 130 mm. Inset, 65 μm.(H and I) Elevated MFGE8 (H) in 5-month-old P301S-tau brains. Lysatesfrom cortex(Ctx), brain stem (BS), and cerebellum(Cb) of 5-month-old C57, 5-month-old P301S-tau, and 5-month-old Alz17 mice probed with anti-MFGE8; MFGE8 KO brain lysate isnegative control. rec, recombinant mouse MFGE8. (I) Densitometry of MFGE8 expression normalized to beta -actin. Significantlyhigher MFGE8 expression in P301S versus C57BU6 Ctx (**p < 0.01), BS (****p < 0.0001), Cb (**p < 0.01), andP301SversusALz17BS (****p < 0.0001). Mean ± SD, n = 3 independent preparations, two-way ANOVA, Bonferronicorrected.(J) Elevated MFGE8 expression in brain extracts from cortex (Ctx) of FTDP-17T patients with two differentMAPT mutations (P301L, +3) or sporadic Pick’s disease but not in extracts from patients with C9orf72hexanucleotide expansions and TDP-43 aggregates. No expression is found in the cerebellum, where tau pathology is absent in allcases. Human milk MFGE8 is a positive control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30134156), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human MFG-E8 by Western Blot MFG-E8 deficiency impairs wound closure and wound angiogenesis.a The serum levels of MF-E8 in HCs (n = 30), diabetes (n = 33), and DFU patients (n = 25) were detected with ELISA. b The serum levels of MFG-E8 in normal or diabetic mice (n = 6) post-wounding for 0, 3, 7, 14 days were determined by ELISA. c Western blot analysis of MFG-E8 expression in skin tissues of normal or diabetic mice after wound for 3 days, GAPDH as a loading control. d The serum fed glucose levels of WT (n = 24) or Mfge8−/− mice (n = 28) when treated with vehicle or STZ for 28 days were measured. e Representative digital imaging of wounds from age-matched diabetic WT or Mfge8−/− mice postwounding up to day 14. f Wound closures kinetics of diabetic WT and Mfge8−/− mice (n = 6). g Representative images of H&E-stained skin tissues from WT or Mfge8−/− mice injected with vehicle or STZ after wound for 3 days. h The amount of CD31+ epithelial cells (ECs) and alpha -smooth muscle actin ( alpha -SMA+) pericytes in wound skins of WT or Mfge8−/− mice treated with vehicle or STZ at day 14 after wound. i Quantification of alpha -SMA+ vessels in five random microscopic fields in n = 6 mice per group was performed. For all experiments, data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32963812), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human MFG-E8 by Western Blot MFGE8 and NO Are Produced and Secreted by Co-cultured BV2 Microglia Only When Co-cultured in Contact with pFTAA+Neurons(A and B) Representative blot (A) ofMFGE8 in BV2 cells lysates cultured alone or co-cultured in direct contact, or via atranswell, with DRG neurons from 5-month-old P301S-tau, 5-month-old C57, 5-month-old Alz17 mice, or 2-month-old P301S mice.Asterisk: either another isoform ofMFGE8 or a breakdown product. (B) Densitometry of MFGE8 expression normalized to beta -actin; significantly more MFGE8 is produced only in contact co-cultures containing pFTAA+ neurons. Mean± SD, n = 3 independent experiments (*p < 0.05), twoway ANOVA, Dunnett’s correction.(C and D) Elevated MFGE8 (C) or NO (D) in medium conditioned by BV2 cells co-cultured in contact with DRG neurons from5-month-old P301S mice compared with 5-month-old Alz17, 5-month- old C57, or 2-month-old P301S mice (MFGE8: ****p < 0.0001versus all; NO: ****p < 0.0001 versus 5-month-old C57 or 2-month-old P301S mice; ***p < 0.001 versus 5-month-oldAlz17 mice). N.D., none detected. Mean ± SD, n = 3 independent experiments, two-way ANOVA, Bonferroni corrected.(E and F) Elevated MFGE8 (E) or NO (F) in medium conditioned by primary microglia from C57 mice co-cultured in contactwith DRG neurons from 5-month-old P301S mice; microglia from MFGE8 KO mice are negative controls. Mean ± SD, n = 3independent microglial preparations, one-way ANOVA, **p < 0.01 (MFGE8), *p < 0.05 (NO).(G) Increased MFGE8 immunostaining intensity in frontal motor cortex of 5-month-old P301S mice compared with 5-month-oldC57 mice; MFGE8 KO mouse is negative control; 25 μm section at inter- aural 5.12 mm, bregma 1.32 mm; brown, DAB; blue,cresyl violet. Scale bar, 130 mm. Inset, 65 μm.(H and I) Elevated MFGE8 (H) in 5-month-old P301S-tau brains. Lysatesfrom cortex(Ctx), brain stem (BS), and cerebellum(Cb) of 5-month-old C57, 5-month-old P301S-tau, and 5-month-old Alz17 mice probed with anti-MFGE8; MFGE8 KO brain lysate isnegative control. rec, recombinant mouse MFGE8. (I) Densitometry of MFGE8 expression normalized to beta -actin. Significantlyhigher MFGE8 expression in P301S versus C57BU6 Ctx (**p < 0.01), BS (****p < 0.0001), Cb (**p < 0.01), andP301SversusALz17BS (****p < 0.0001). Mean ± SD, n = 3 independent preparations, two-way ANOVA, Bonferronicorrected.(J) Elevated MFGE8 expression in brain extracts from cortex (Ctx) of FTDP-17T patients with two differentMAPT mutations (P301L, +3) or sporadic Pick’s disease but not in extracts from patients with C9orf72hexanucleotide expansions and TDP-43 aggregates. No expression is found in the cerebellum, where tau pathology is absent in allcases. Human milk MFGE8 is a positive control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30134156), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human MFG-E8 by Western Blot The activation of NLRP3 inflammasome was aggravated in MFG-E8-deficient mice.a The infiltrated active caspase-1+ and IL-1 beta + macrophages in dermis of wound skins from WT or Mfge8−/− mice (n = 6) treated with vehicle or STZ, post-wounding at day 3, were determined with immunofluorescence. b Quantification of the expression of active caspase-1, caspase-1, and MFG-E8 in wound skin tissue from WT or Mfge8−/− mice (n = 6) treated with vehicle or STZ at day 3 post-wounding, beta -actin as loading control. c Calculation of the ratio of active caspase-1/caspase-1 in wound skin tissues from each group mice. d Infiltration of death cells into wound skin tissues of WT or Mfge8−/− mice (n = 6) post-wounding at day 3 was identified by TUNEL. Representative images were shown, original magnification ×200. e The average TUNEL-positive cells were counted at more than five random microscopic fields. For all experiments, data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32963812), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Human MFG-E8 by Western Blot MFGE8 and NO Are Produced and Secreted by Co-cultured BV2 Microglia Only When Co-cultured in Contact with pFTAA+Neurons(A and B) Representative blot (A) ofMFGE8 in BV2 cells lysates cultured alone or co-cultured in direct contact, or via atranswell, with DRG neurons from 5-month-old P301S-tau, 5-month-old C57, 5-month-old Alz17 mice, or 2-month-old P301S mice.Asterisk: either another isoform ofMFGE8 or a breakdown product. (B) Densitometry of MFGE8 expression normalized to beta -actin; significantly more MFGE8 is produced only in contact co-cultures containing pFTAA+ neurons. Mean± SD, n = 3 independent experiments (*p < 0.05), twoway ANOVA, Dunnett’s correction.(C and D) Elevated MFGE8 (C) or NO (D) in medium conditioned by BV2 cells co-cultured in contact with DRG neurons from5-month-old P301S mice compared with 5-month-old Alz17, 5-month- old C57, or 2-month-old P301S mice (MFGE8: ****p < 0.0001versus all; NO: ****p < 0.0001 versus 5-month-old C57 or 2-month-old P301S mice; ***p < 0.001 versus 5-month-oldAlz17 mice). N.D., none detected. Mean ± SD, n = 3 independent experiments, two-way ANOVA, Bonferroni corrected.(E and F) Elevated MFGE8 (E) or NO (F) in medium conditioned by primary microglia from C57 mice co-cultured in contactwith DRG neurons from 5-month-old P301S mice; microglia from MFGE8 KO mice are negative controls. Mean ± SD, n = 3independent microglial preparations, one-way ANOVA, **p < 0.01 (MFGE8), *p < 0.05 (NO).(G) Increased MFGE8 immunostaining intensity in frontal motor cortex of 5-month-old P301S mice compared with 5-month-oldC57 mice; MFGE8 KO mouse is negative control; 25 μm section at inter- aural 5.12 mm, bregma 1.32 mm; brown, DAB; blue,cresyl violet. Scale bar, 130 mm. Inset, 65 μm.(H and I) Elevated MFGE8 (H) in 5-month-old P301S-tau brains. Lysatesfrom cortex(Ctx), brain stem (BS), and cerebellum(Cb) of 5-month-old C57, 5-month-old P301S-tau, and 5-month-old Alz17 mice probed with anti-MFGE8; MFGE8 KO brain lysate isnegative control. rec, recombinant mouse MFGE8. (I) Densitometry of MFGE8 expression normalized to beta -actin. Significantlyhigher MFGE8 expression in P301S versus C57BU6 Ctx (**p < 0.01), BS (****p < 0.0001), Cb (**p < 0.01), andP301SversusALz17BS (****p < 0.0001). Mean ± SD, n = 3 independent preparations, two-way ANOVA, Bonferronicorrected.(J) Elevated MFGE8 expression in brain extracts from cortex (Ctx) of FTDP-17T patients with two differentMAPT mutations (P301L, +3) or sporadic Pick’s disease but not in extracts from patients with C9orf72hexanucleotide expansions and TDP-43 aggregates. No expression is found in the cerebellum, where tau pathology is absent in allcases. Human milk MFGE8 is a positive control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30134156), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MFG-E8
Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C-terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).
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- Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
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- Hanayama, R. et al. (2002) Nature 417:182.
- Ait-Oufella, H. et al. (2007) Circulation 115:2168.
- Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
- Hanayama, R. et al. (2004) Science 304:1147.
- Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
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- Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
- Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
Product Datasheets
Citations for Mouse MFG-E8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models
Authors: Sokolova, D;Ghansah, SA;Puletti, F;Georgiades, T;De Schepper, S;Zheng, Y;Crowley, G;Wu, L;Rueda-Carrasco, J;Koutsiouroumpa, A;Muckett, P;Freeman, OJ;Khakh, BS;Hong, S;
bioRxiv : the preprint server for biology
Species: Transgenic Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Inter-axonal molecular crosstalk via Lumican proteoglycan sculpts murine cervical corticospinal innervation by distinct subpopulations
Authors: Y Itoh, V Sahni, SJ Shnider, H McKee, JD Macklis
Cell Reports, 2023-03-17;42(3):112182.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
Small extracellular vesicles from Ptpn1-deficient macrophages alleviate intestinal inflammation by reprogramming macrophage polarization via lactadherin enrichment
Authors: D Han, D Lu, S Huang, J Pang, Y Wu, J Hu, X Zhang, Y Pi, G Zhang, J Wang
Redox Biology, 2022-11-28;58(0):102558.
Species: Transgenic Mouse
Sample Types: In Vivo
Applications: In Vivo -
Medin co-aggregates with vascular amyloid-beta in Alzheimer's disease
Authors: J Wagner, K Degenhardt, M Veit, N Louros, K Konstantou, A Skodras, K Wild, P Liu, U Obermüller, V Bansal, A Dalmia, LM Häsler, M Lambert, M De Vleesch, HA Davies, J Madine, D Kronenberg, R Feederle, D Del Turco, KPR Nilsson, T Lashley, T Deller, M Gearing, LC Walker, P Heutink, F Rousseau, J Schymkowit, M Jucker, JJ Neher
Nature, 2022-11-16;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways
Authors: Minmin Cai, Liufang Sheng
Stem Cells International
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Inflammatory Role of Milk Fat Globule–Epidermal Growth Factor VIII in Age‐Associated Arterial Remodeling
Authors: Leng Ni, Lijuan Liu, Wanqu Zhu, Richard Telljohann, Jing Zhang, Robert E. Monticone et al.
Journal of the American Heart Association
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The Potential of Bovine Colostrum-Derived Exosomes to Repair Aged and Damaged Skin Cells
Authors: G Han, H Kim, DE Kim, Y Ahn, J Kim, YJ Jang, K Kim, Y Yang, SH Kim
Pharmaceutics, 2022-01-27;14(2):.
Species: Bovine
Sample Types: Milk Exosomes
Applications: Western Blot -
MFG-E8 (LACTADHERIN): a novel marker associated with cerebral amyloid angiopathy
Authors: P Marazuela, M Solé, A Bonaterra-, J Pizarro, J Camacho, E Martínez-S, HB Kuiperij, MM Verbeek, AM de Kort, FHBM Schreuder, CJM Klijn, L Castillo-R, O Pancorbo, D Rodríguez-, F Pujadas, P Delgado, M Hernández-
Acta neuropathologica communications, 2021-09-16;9(1):154.
Species: Mouse, Transgenic Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-&kappaB pathway
Authors: Y Lu, L Liu, J Pan, B Luo, H Zeng, Y Shao, H Zhang, H Guan, D Guo, C Zeng, R Zhang, X Bai, H Zhang, D Cai
Cell Death & Disease, 2021-05-25;12(6):533.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
MFG-E8 Plays an Important Role in Attenuating Cerulein-Induced Acute Pancreatitis in Mice
Authors: HF Bu, S Subramania, H Geng, X Wang, F Liu, PM Chou, C Du, IG De Plaen, XD Tan
Cells, 2021-03-25;10(4):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain
Authors: C Madore, Q Leyrolle, L Morel, M Rossitto, AD Greenhalgh, JC Delpech, M Martinat, C Bosch-Bouj, J Bourel, B Rani, C Lacabanne, A Thomazeau, KE Hopperton, S Beccari, A Sere, A Aubert, V De Smedt-P, C Lecours, K Bisht, L Fourgeaud, S Gregoire, L Bretillon, N Acar, NJ Grant, J Badaut, P Gressens, A Sierra, O Butovsky, ME Tremblay, RP Bazinet, C Joffre, A Nadjar, S Layé
Nature Communications, 2020-11-30;11(1):6133.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Cardiomyocytes capture stem cell-derived, anti-apoptotic microRNA-214 via clathrin-mediated endocytosis in acute myocardial infarction
Authors: S Eguchi, M Takefuji, T Sakaguchi, S Ishihama, Y Mori, T Tsuda, T Takikawa, T Yoshida, K Ohashi, Y Shimizu, R Hayashida, K Kondo, YK Bando, N Ouchi, T Murohara
J. Biol. Chem., 2019-06-19;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot -
TGF beta -Signaling and FOXG1-Expression Are a Hallmark of Astrocyte Lineage Diversity in the Murine Ventral and Dorsal Forebrain
Authors: Stefan Christopher Weise, Alejandro Villarreal, Stefanie Heidrich, Fariba Dehghanian, Christian Schachtrup, Sigrun Nestel et al.
Frontiers in Cellular Neuroscience
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Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia
Authors: J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin
Cell Rep, 2018-08-21;24(8):1939-1948.e4.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Obesity and Insulin Resistance Promote Atherosclerosis through an IFN?-Regulated Macrophage Protein Network
Authors: CA Reardon, A Lingaraju, KQ Schoenfelt, G Zhou, C Cui, H Jacobs-El, I Babenko, A Hoofnagle, D Czyz, H Shuman, T Vaisar, L Becker
Cell Rep, 2018-06-05;23(10):3021-3030.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release
Authors: Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura
JCI Insight, 2018-04-19;3(8):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Anti-HER2 scFv-directed extracellular vesicle-mediated mRNA-based gene delivery inhibits growth of HER2-positive human breast tumor xenografts by prodrug activation
Authors: JH Wang, AV Forterre, J Zhao, DO Frimannsso, A Delcayre, TJ Antes, B Efron, SS Jeffrey, MD Pegram, AC Matin
Mol. Cancer Ther., 2018-02-26;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Developmental Emergence of Adult Neural Stem Cells as Revealed by Single-Cell Transcriptional Profiling
Authors: SA Yuzwa, MJ Borrett, BT Innes, A Voronova, T Ketela, DR Kaplan, GD Bader, FD Miller
Cell Rep, 2017-12-26;21(13):3970-3986.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Spherical nucleic acid targeting microRNA-99b enhances intestinal MFG-E8 gene expression and restores enterocyte migration in lipopolysaccharide-induced septic mice
Sci Rep, 2016-08-19;6(0):31687.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Molecular Mechanisms Underlying the Regulation of the MFG-E8 Gene Promoter Activity in Physiological and Inflammatory Conditions
Authors: Xiao Wang, Heng-Fu Bu, Shirley X L Liu, Isabelle G. De Plaen, Xiao-Di Tan
Journal of Cellular Biochemistry
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MFG-E8 regulates microglial phagocytosis of apoptotic neurons.
Authors: Fuller AD, Van Eldik LJ
J Neuroimmune Pharmacol, 2008-08-01;3(4):246-56.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Mfge8 promotes obesity by mediating the uptake of dietary fats and serum fatty acids.
Authors: Khalifeh-Soltani A, McKleroy W, Sakuma S et al.
Nat. Med.
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Clusterin in the mouse epididymis: possible roles in sperm maturation and capacitation.
Authors: Saewu A, Kadunganattil S, Raghupathy R et al.
Reproduction.
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Allograft inflammatory factor-1 supports macrophage survival and efferocytosis and limits necrosis in atherosclerotic plaques
Authors: Lander Egaña-Gorroño, Prameladevi Chinnasamy, Isabel Casimiro, Vanessa M. Almonte, Dippal Parikh, Gustavo H. Oliveira-Paula et al.
Atherosclerosis
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Immunohistochemistry/fluorescence (Frozen sections).
10 µm cryostat sections from fresh frozen brain sample. Post-fixation with 4% formaldehyde for 15min..
Blocking: PBS + 1% BSA +1% ovalbumin +10% normal donkey serum +0.3% LDAO +0.3M Glycine.
Incubation buffer: PBS + 1% BSA +1% ovalbumin +10% normal donkey serum.
Primary AB: goat anti-mouse MFG-E8 (#AF2805) 2 µg/ml, 2h at room temperature.
DRAQ5 5µM (nuclei marker) together with primary AB.
Secondary AB: Donkey anti-goat/AlexaFluor488 4 µg/ml, 1h at RT.
Autofluorescence suppression: 4mM CuSO4 in 50mM ammoniumacetate buffer, pH 5, 30min at RT.
Imaging: Evident SlideView VS200 slide scanner with X-Cite NOVEM illumination.
Dilution: 1:1000
Secondary used: Goat IgG Horseradish Peroxidase-conjugated Antibody from R&D Systems.
