Mouse MFRP Biotinylated Antibody

Catalog #: BAF3445 Datasheet / COA / SDS

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BAF3445

All MFRP Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MFRP Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MFRP in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) MFRP is observed, 15% cross-reactivity with recombinant mouse (rm) Frizzled-4 is observed, 10% cross-reactivity with rmFrizzled-1, rmFrizzled-8, and rmFrizzled-10 is observed, 5% cross-reactivity with rhFrizzled-5 is observed, and 1% cross-reactivity with rmFrizzled-3, rmFrizzled-6, and rmFrizzled-7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MFRP
Gln91-Pro584
Accession # Q8K480

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse MFRP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFRP

MFRP is a type II transmembrane protein that is related to a small family of soluble frizzled-related proteins. It is the product of a dicistronic mRNA transcript that codes for two proteins; MFRP and C1qTNF5. Mouse MFRP is 584 amino acids (aa) in length. Its extracellular domain (ECD) of 494 aa contains two CUB and two LDLR class A segments, plus one frizzled domain. At least two splice variants are known in mouse. In isoform 2, amino acid residues 425‑431 of isoform 1 is substituted with Lys. Mouse MFRP ECD shares 72% aa identity with human MFRP ECD. In mouse retinal degeneration 6, MFRP is mutated.

Long Name

Membrane Frizzled-related Protein

Entrez Gene IDs

83552 (Human); 259172 (Mouse)

Alternate Names

MCOP5; membrane frizzled-related protein; Membrane-type frizzled-related protein; MFRP; MGC32938; NNO2; rd6