Mouse MHC class II (I-A/I-E) Fluorescein-conjugated Antibody

Name: Mouse MHC class II (I-A/I-E) Fluorescein-conjugated Antibody
Brand: R&D Systems
SKU: FAB6118F
Price: 309 USD

Catalog #: FAB6118F Datasheet / COA / SDS

Discontinued Product

FAB6118F has been discontinued.

View all MHC class II (I-A/I-E) products.

Detection of MHC class II (I‑A/I‑E) in Mouse Splenocytes by Flow Cytometry.

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Mouse MHC class II (I-A/I-E) Fluorescein-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MHC class II (I‑A/I‑E).

Source

Monoclonal Rat IgG_2B Clone # M5/114.15.2

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Activated C57BL/6 mouse spleen cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MHC class II (I‑A/I‑E) in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse MHC class II (I-A/I-E) Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB6118F) or (B) Rat IgG_2BFluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MHC class II (I-A/I-E)

The M5/114.15.2 monoclonal antibody recognizes a homologous region of MHC class II associated I-A and I-E antigens (1). It binds a region shared by I-A^b, I-A^d, I-A^q, I-E^d and I-E^k MHC class II antigens, which are expressed on antigen presenting cells (2).

References

Bhattacharya, A. et al. (1981) J. Immunol. 127:2448.
Watts, C. et al. (1997) Ann. Rev. Immunol. 15:821.

Long Name

Major Histocompatibility Complex Class II IA and IE

Entrez Gene IDs

14968 (Mouse)

Alternate Names

E-alpha-f;H-2 class II histocompatibility antigen, E-K alpha chain;H-2 class II histocompatibility antigen, E-U alpha chain;H-2E;H2-Ea;H-2Ea;H2-Ea-ps;I;Ia;Ia3;Ia-3;I-E;I-Ealpha;MHC-H2-Ea; H2-Ea; MHC class II (I-A/I-E); MHC class II IA/IE