Mouse MIXL1 Antibody

Catalog #: MAB8099 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8099
MAB8099-SP

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Mouse MIXL1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MIXL1 in ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 868132

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse MIXL1
Lys147-Phe231
Accession # Q9WVI0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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MIXL1 in D3 Mouse Embryonic Stem Cells. MIXL1 was detected in immersion fixed D3 mouse embryonic stem cells differentiated by LIF withdrawal for 3 days using Rat Anti-Mouse MIXL1 Monoclonal Antibody (Catalog # MAB8099) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei in a subset of differentiating cells. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIXL1

MIXL1 (Mix-like 1) is a 36 kDa member of the paired-like homeodomain protein family. It is expressed in the primitive streak of the gastrulating embryo, and marks cells destined to form mesoderm and endoderm. It is also expressed in differentiating embryonic stem cells, immature B and T lymphocytes during normal hematopoiesis, and several B, T or myeloid lineage acute leukemias. The 231 amino acid (aa) human MIXL1 contains a homeobox domain at aa 86-145. The mouse MIXL1 immunogen (aa 147-231) shares 72% and 91% aa sequence identity with human and rat MIXL1, respectively.

Long Name

Mix1 Homeobox-like 1

Entrez Gene IDs

83881 (Human); 27217 (Mouse)

Alternate Names

hMix; homeobox protein MIXL1; Homeodomain protein MIX; MGC138179; MILD1; Mix.1 homeobox-like protein; Mix1 homeobox (Xenopus laevis)-like 1; Mix1 homeobox-like 1 (Xenopus laevis); MIX1 homeobox-like protein 1; MIXL1; Mix-like homeobox protein 1; MIXLMIX