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Mouse MUP-1 Antibody

Catalog #: MAB6560
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6560
MAB6560-SP
MUP‑1 in NCTC 1469 Mouse Cell Line.
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Citations (1)
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Mouse MUP-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MUP-1 in direct ELISAs.
Source
Monoclonal Mouse IgM Clone # 663323
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse MUP-1 synthetic peptide
Accession # NP_112465
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry MUP-1 antibody in NCTC 1469 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

MUP‑1 in NCTC 1469 Mouse Cell Line. MUP-1 was detected in immersion fixed NCTC 1469 mouse liver cell line using Mouse MUP-1 Monoclonal Antibody (Catalog # MAB6560) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MUP-1

Major urinary protein 1 (MUP-1) is a 20 kDa lipocalin family glycoprotein that is secreted by the liver into the circulation and then excreted in the urine of male mice. Its beta -barrel structure with an internal hydrophobic cavity enables MUP-1 to serve as a carrier for a variety of pheromones. In the circulation, MUP-1 promotes energy expenditure, mitochondrial biogenesis, and oxidative function, and it inhibits hepatic glucose production, triglyceride accumulation, glucose intolerance, and insulin resistance. MUP-1 is downregulated in the liver and circulation of diabetic obese mice.

Long Name
Major Urinary Protein 1
Entrez Gene IDs
17840 (Mouse)
Alternate Names
Ltn-1; Lvtn-1; major urinary protein 1; major urinary protein 10; major urinary protein 7; MUP 1; Mup1 major urinary protein 1; MUP1; MUP-1; Mup10; Mup7; Mup-a; Up-1

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Citation for Mouse MUP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hepatic Tumor Formation in Adult Mice Developmentally Exposed to Organotin
    Authors: Tiffany A. Katz, Sandra L. Grimm, Akhilesh Kaushal, Jianrong Dong, Lindsey S. Treviño, Rahul K. Jangid et al.
    Environmental Health Perspectives

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