Mouse NAIP Antibody

Catalog #: AF7608 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7608
AF7608-SP

Detection of Mouse NAIP by Western Blot.

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Mouse NAIP Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse NAIP in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse NAIP2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse NAIP
Lys783-Ser856
Accession # Q9QWK5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse NAIP by Western Blot. Western blot shows lysates of mouse liver tissue and mouse colon tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse NAIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7608) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NAIP at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NAIP

NAIP-1 (Neuronal Apoptosis Inhibitory Protein 1; also Naip and Birc1) is a 150-160 kDa member of both the NLR/PRR and IAP family of proteins. It is widely, but not ubiquitously expressed, and is found in adipocytes, macrophages, neurons, renal proximal and distal convoluted tubules, and nonproliferative intestinal epithelium. NAIP-1 plays a key regulatory role in caspase activation. It promotes caspase-1 activation, blocks caspase-3 and -7 activation, and by binding neuronal hippocalcin, inhibits caspase-3-independent apoptosis. Mouse NAIP-1 is 1403 amino acids (aa) in length. It contains three consecutive N-terminal BIR (Baculovirus Inhibition of apoptosis Repeat) domains (aa 63-346) and one NACHT region (aa 464-758). The BIR #2 domain is posited to bind both caspase-3 and -7, while the BIR domain #3 likely interacts with hippocalcin. NAIP-1 undergoes proteolysis, generating an N-terminal 100 kDa fragment (likely aa 1- ≈ 869) and a 60 kDa C-terminal fragment (likely aa 870-1403). The C-terminal fragment undergoes further processing to generate a 25 kDa and 35 kDa fragment. There are at least seven numbered NAIP genes in mouse that have arisen by gene duplication. Over the aa range 783-856, they share less than 60% aa sequence identity with NAIP-1. Over the same aa range, mouse NAIP-1 shares 54% aa sequence identity with the only human NAIP gene.

Long Name

Neuronal Apoptosis Inhibitor Protein

Entrez Gene IDs

4671 (Human); 17940 (Mouse); 191568 (Rat)

Alternate Names

baculoviral IAP repeat-containing 1; BIRC1; BIRC1baculoviral IAP repeat-containing protein 1; FLJ18088; FLJ42520; FLJ58811; NAIP; Neuronal apoptosis inhibitory protein; NLR family, apoptosis inhibitory protein; NLR family, BIR domain containing 1; NLRB1; nucleotide-binding oligomerization domain, leucine rich repeat and BIR domaincontaining 1; psi neuronal apoptosis inhibitory protein; psiNAIP