Mouse Neogenin Biotinylated Antibody

Catalog #: BAF1079 Datasheet / COA / SDS

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BAF1079

All Neogenin Antibodies

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Mouse Neogenin Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Neogenin in Western blots. In this format, approximately 5% cross-reactivity with recombinant mouse DCC is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Neogenin
Ala42-Ile1033 with a Asp442-Leu461 deletion
Accession # NP_032710

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Neogenin Fc Chimera (Catalog # 1079-NE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neogenin

Neogenin (NEO) is a type I transmembrane protein that is crucial for axonal guidance and neuronal migration. It is also involved in regulating differentiation programs in many embryonic and adult tissues (1). Mouse NEO is widely expressed in adult tissues and is expressed throughout the mid to late stages of gestation, in both neuronal and non-neuronal tissues. It is a member of the immunoglobulin (Ig) superfamily and is closely related to deleted in colorectal cancer (DCC). Mouse NEO cDNA encodes a 1493 amino acid residue (aa) precursor with a putative 36 aa signal peptide, a 1100 aa extracellular domain with six Ig-like C2 type domains and three fibronectin type III domains, a 21 aa transmembrane domain and a 345 aa cytoplasmic domain. At least five isoforms are produced in mice by alternative splicing. Mouse NEO shares 96%, 93% and 86% aa sequence identity with rat, human and chicken NEO, respectively. It also has 46% and 29% sequence homology with mouse DCC and C. elegans UNC40, a homolog of DCC. NEO and DCC, together with the UNC5 family of type I transmembrane proteins, are receptors for the netrin/UNC6 family of laminin-related bifunctional guidance molecules that both attract some axons and repel others (2, 3). In mouse, at least five netrins (netrin‑1, ‑3, ‑4, G1 and G2) have been identified (3‑5). Mouse netrin-1 and netrin-3 have been shown to be ligands for mouse NEO.

References

Keeling, S.L. et al. (1997) Oncogene 15:691.
Hong, K. et al. (1999) Cell 97:927.
Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
Nakashiba, T. et al. (2000) J. Neurosci. 20:6540.
Nakashiba, T. et al. (2002) Mech. Dev. 111:47.

Entrez Gene IDs

4756 (Human); 18007 (Mouse)

Alternate Names

HsT17534; IGDCC2DKFZp547B146; Immunoglobulin superfamily DCC subclass member 2; NEO; NEO1; neogenin (chicken) homolog 1; neogenin 1; neogenin homolog 1; Neogenin; NGN; NGNDKFZp547A066

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Product Datasheet

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SDS

Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order toobtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.