Mouse Nephronectin Antibody Summary
Asp20-Cys561
Accession # AAH68308
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Nephronectin in Mouse Pancreas. Nephronectin was detected in immersion fixed paraffin-embedded sections of mouse pancreas using Goat Anti-Mouse Nephronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4298) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nephronectin
Nephronectin, also named POEM (preosteoblast EGF repeat protein with MAM domain) is a 70‑90 kDa extracellular matrix protein that is a ligand for integrin alpha 8 beta 1 (1, 2). The 561 amino acid (aa) mouse Nephronectin (isoform b) contains a 19 aa signal sequence, a matrilin-type vWA domain, two calcium-binding EGF-like domains, a potential N-linked glycosylation site, a pro/ser/thr-rich mucin-like region, an RGD integrin binding motif, and a MAM domain. These features are often present in matrix proteins that, like Nephronectin, mediate cell adhesion and spreading (1, 2). Isoform a includes a 17 aa insertion between aa 58 and 59 that is missing in isoform b. Mouse Nephronectin shows 88%, 92%, 85% and 77% aa identity with human, rat, bovine and opossum Nephronectin, respectively. It is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, and around developing bone, teeth, and muscle (1, 2). Pre-osteoblast cells produce Nephronectin, but down‑regulate it as they differentiate (1). It is also expressed in the Wolffian duct and ureteric bud basement membranes in the developing kidney, where it is colocalizes, and forms an in-vivo complex with integrin alpha 8 beta 1 (2). Since deletion of integrin alpha 8 beta 1 results in renal agenesis, Nephronectin has been proposed to be a critical integrin alpha 8 beta 1 ligand during Nephrogenesis (2, 3).
Product Datasheets
Citations for Mouse Nephronectin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Cell Types Promoting Goosebumps Form a Niche to Regulate Hair Follicle Stem Cells
Authors: Yulia Shwartz, Meryem Gonzalez-Celeiro, Chih-Lung Chen, H. Amalia Pasolli, Shu-Hsien Sheu, Sabrina Mai-Yi Fan et al.
Cell
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Nephronectin Expression Is Up-Regulated by BMP-2
Authors: Tamaki Kurosawa, Atsushi Yamada, Dai Suzuki, Naoko Morimura, Yoshiyuki Sasagane, Hiroyuki Itabe et al.
Biological & Pharmaceutical Bulletin
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Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages
Authors: Alicia Bellomo, Isabelle Mondor, Lionel Spinelli, Marine Lagueyrie, Benjamin J. Stewart, Nicolas Brouilly et al.
Immunity
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FGF-2 suppresses expression of nephronectin via JNK and PI3K pathways
Authors: Kato T, Yamada A, Ikehata M et al.
FEBS Open Bio
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Nephronectin-integrin alpha8 signaling is required for proper migration of periocular neural crest cells during chick corneal development
Authors: J Ma, L Bi, J Spurlin, P Lwigale
Elife, 2022-03-03;11(0):.
Species: Chicken
Sample Types: Whole Cells
Applications: Stimulation -
A cell-ECM mechanism for connecting the ipsilateral eye to the brain
Authors: J Su, U Sabbagh, Y Liang, L Olejníková, KG Dixon, AL Russell, J Chen, YA Pan, JW Triplett, MA Fox
Proceedings of the National Academy of Sciences of the United States of America, 2021-10-19;118(42):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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