Mouse Netrin-G2a Antibody

Catalog #: MAB27441 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB27441
MAB27441-SP

All Netrin-G2a Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Netrin-G2a Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Netrin-G2a in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) Netrin-G1a, rmNetrin-1, or rmNetrin-4 is observed.

Source

Monoclonal Rat IgG_2A Clone # 349724

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Netrin-G2a
Asp18-Arg504
Accession # NP_598007

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Netrin-G2a

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of mouse brain (hippocampus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-G2a

Mouse Netrin-G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin-G2a is approximately 485 aa in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF-like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59 aa and 34 aa between EGF-like motifs 1 and 2 generate Netrin-G2b and Netrin-G2c, respectively. Mature mouse Netrin-G2a shares 96% and 99% aa identity with human Netrin-G2 and the equivalent region in rat Netrin-G2b, respectively.

Entrez Gene IDs

84628 (Human); 171171 (Mouse)

Alternate Names

bA479K20.1;LHLL9381;Lmnt2;NEDBASH;NTNG1;NTNG2; Lmnt2a; NetrinG2a; Netrin-G2a; NTG2a

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.