Mouse Neutrophil Elastase/ELA2 Antibody Summary
Met1-Arg260
Accession # Q3UP87
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Neutrophil Elastase/ELA2 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB4517) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Neutrophil Elastase/ELA2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neutrophil Elastase/ELA2
Neutrophil Elastase (ELA2), also known as Leukocyte Elastase, Bone marrow serine protease, Medullasin and ELANE, is a 25-30 kDa serine protease belonging to the peptidase S1 family. ELA2 is secreted by neutrophils during inflammation, it destroys bacteria and host tissue. ELA2 function is the degradation of many extracellular matrix macromolecules. ELA2 is localized to keratohyalin granules, where it was found to directly participate in pro-filaggrin processing. When expressed aberrantly, ELA2 can cause emphysema or emphysematous changes, it involves breakdown of the lung structure and increased airspaces. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity.
Product Datasheets
Citations for Mouse Neutrophil Elastase/ELA2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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S100A9 is indispensable for survival of pneumococcal pneumonia in mice
Authors: Ostermann, L;Seeliger, B;David, S;Flasche, C;Maus, R;Reinboth, MS;Christmann, M;Neumann, K;Brand, K;Seltmann, S;Bühling, F;Paton, JC;Roth, J;Vogl, T;Viemann, D;Welte, T;Maus, UA;
PLoS pathogens
Species: Mouse
Sample Types: BALF
Applications: Western Blot -
Neutrophil Extracellular Traps Mediate Acute Liver Failure in Regulation of miR-223/neutrophil Elastase Signaling in Mice
Authors: D Ye, J Yao, W Du, C Chen, Y Yang, K Yan, J Li, Y Xu, S Zang, Y Zhang, X Rong, R Zhang, A Xu, J Guo
Cellular and Molecular Gastroenterology and Hepatology, 2022-06-02;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells, Whole Tissue
Applications: ICC, IHC, Western Blot -
A novel in vivo model for extracellular vesicle-induced emphysema
Authors: C Margaroli, MC Madison, L Viera, DW Russell, A Gaggar, KR Genschmer, JE Blalock
JCI Insight, 2022-01-25;0(0):.
Species: Mouse
Sample Types: Extracellular Vesicles
Applications: Flow Cytometry -
IL-1? activation in response to Staphylococcus aureus lung infection requires inflammasome-dependent and independent mechanisms
Authors: S Pires, D Parker
Eur. J. Immunol., 2018-08-19;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Midkine drives cardiac inflammation by promoting neutrophil trafficking and NETosis in myocarditis
Authors: Ludwig T. Weckbach, Ulrich Grabmaier, Andreas Uhl, Sebastian Gess, Felicitas Boehm, Annette Zehrer et al.
Journal of Experimental Medicine
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- loaded 10, 20, 30ug of mouse kidney tissue
- blocked and diluted ELA2 Ab in 5% milk (1:1000)
- not sure what the HMW bands is though
