Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit

Name: Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit
Brand: R&D Systems
SKU: MELA20
Rating: 5 (1 reviews)

Catalog #: MELA20
2 Citations 1 Review Datasheet / COA / SDS

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MELA20

Mouse Neutrophil Elastase/ELA2 Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Lysates (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

3.37 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse ELA2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine^® Mouse Neutrophil Elastase/ELA2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure ELA2 in cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse ELA2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse ELA2. Results obtained using natural mouse ELA2 showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ELA2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	104	254	735	96.9	238	709
Standard Deviation	1.28	3.64	15.7	9.39	17.4	43.6
CV%	1.2	1.4	2.1	9.7	7.3	6.1

Recovery

The recovery of mouse ELA2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	96	90-102
Cell Lysis Buffer (n=3)	96	91-97
EDTA Plasma (n=4)	108	95-125
Heparin Plasma (n=4)	108	101-118
Serum (n=4)	112	104-122

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse ELA2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Mouse Neutrophil Elastase/ELA2 Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Entrez Gene IDs:

1991 (Human); 50701 (Mouse)

Alternate Names:

Bone marrow serine protease; EC 3.4.21; EC 3.4.21.37; ELA2; ELA2granulocyte-derived elastase; ELANE; elastase 2, neutrophil; elastase, neutrophil expressed; Elastase-2; GE; HLEelastase-2; HNE; Human leukocyte elastase; Leukocyte Elastase; Medullasin; NE; Neutrophil Elastase; PMN elastase; PMN-E; polymorphonuclear elastase; SCN1

Related Research Areas

Citations for Mouse Neutrophil Elastase/ELA2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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CD14 Blockade Does Not Improve Outcomes of Deep Vein Thrombosis Following Inferior Vena Cava Stenosis in Mice
Authors: Pandey, N;Kaur, H;Chandaluri, L;Anand, SK;Chokhawala, H;Magdy, T;Stokes, KY;Orr, AW;Rom, O;Dhanesha, N;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Plasma
Pseudomonas aeruginosa-induced nociceptor activation increases susceptibility to infection
Authors: T Lin, D Quellier, J Lamb, T Voisin, P Baral, F Bock, A Schönberg, R Mirchev, G Pier, I Chiu, M Gadjeva
PloS Pathogens, 2021-05-06;17(5):e1009557.
Species: Mouse
Sample Types: Tissue Homogenates