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Mouse NKp46/NCR1 Antibody

Catalog #: AF2225
65 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2225
AF2225-SP
Mouse NKp46/NCR1 Antibody Induces IFN-gamma Secretion in Activated Mouse NK Cells.
6 Images
Product Details
Citations (65)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NKp46/NCR1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) NKp46 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NKp46/NCR1
Glu22-Asn255
Accession # Q8C567
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse NKp46/NCR1 Fc Chimera (Catalog # 2225-NK)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
3-15 µg/mL
See below
Agonist Activity
0.4-2.4 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Agonist Activity Mouse NKp46/NCR1 Antibody Induces IFN-? Secretion antibody in Activated Mouse NK Cells. View Larger

Mouse NKp46/NCR1 Antibody Induces IFN-gamma Secretion in Activated Mouse NK Cells. Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody induces IFN-? secretion in mouse natural killer (NK) cells activated with 25 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) and 25 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML), in a dose-dependent manner, as measured using the Quantikine Mouse IFN-? ELISA Kit (Catalog # MIF00). The ED50 for this effect is typically 0.4-2.4 µg/mL.

Flow Cytometry Detection of NKp46/NCR1 antibody in Mouse DX5/CD49b<SUP>+</SUP>Splenocytes antibody by Flow Cytometry. View Larger

Detection of NKp46/NCR1 in Mouse DX5/CD49b+Splenocytes by Flow Cytometry. Mouse DX5/CD49b+splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunohistochemistry NKp46/NCR1 antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr). View Larger

NKp46/NCR1 in Mouse Spleen. NKp46/NCR1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry/ Immunofluorescence Detection of Mouse NKp46/NCR1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse NKp46/NCR1 by Immunocytochemistry/Immunofluorescence IL-2/Anti-IL-2 Antibody Complex Treatment Triggers NK Cell-Dependent Acute Sensory Loss after Partial Sciatic Nerve Crush(A) Peripheral blood sampled 16 days post-injury in IL-2 complex or IgG control mice. NKp46+DX5+ NK cells (U = 15.00, p = 0.0019); CD3+CD8+ T cells (t = 15.78, p < 0.0001); CD3+CD4+ T cells (t = 9.719, p < 0.0001). Mann-Whitney or Student’s unpaired t test was used.(B) Daily pinprick response. Male wild-type C57BL/6 mice received partial crush of the sciatic nerve on day 0 followed by daily injection of IL-2 complex or IgG control (i.p.) for 4 consecutive days (arrows). Two-way ANOVA was used. Effect of treatment: F(1,315) = 20.69, p < 0.0001. Bonferroni post-test ∗∗p < 0.01 (t = 3.784), ∗∗∗p < 0.001 (t = 4.741).(C) Heatmap showing mean sensitivity to pinprick along the lateral hind paw. Note the broad loss of sensitivity at day 6 in IL-2-complex-treated mice.(D) Area under the curve measurements in IL-2-complex-treated and IgG control mice. Days 1–4: p = 0.9513, t = 0.06181; days 5–10: ∗∗∗p = 0.0083, t = 2.916; days 11–15: p = 0.4354, t = 0.7951. Student’s unpaired t test was used.(E) Effect of IL-2 complex treatment on NKp46-YFP+ cell infiltration to sciatic nerve 6 days after partial crush injury. Sciatic nerve sections (14 μm) were immunolabeled with anti-GFP and beta -tubulin III antibodies. Scale bars, 100 μm. Arrows indicate individual YFP+ cells.(F) Quantification of NKp46-YFP+ cells per square millimeter in images of different regions of the nerve. Two-way ANOVA: effect of IL-2 complex, F(1,16) = 56.31, p < 0.0001; effect of region, F(3,16) = 23.73, p < 0.0001. Bonferroni post-tests: crush, t = 8.062; distal, t = 6.414 ∗∗∗p < 0.001. ns, not significant. n = 3 sections per region, per mouse, per treatment.(G) Photograph of spleens isolated from mice 1 day after final injection of IgG or IL-2 complex.(H) Peripheral blood sampled 16 days post-injury in IL-2-complex-treated wild-type mice, which received either anti-NK1.1 antibody or isotype control. NKp46+DX5+ NK cells (t = 15.37, ∗∗∗p < 0.0001), CD3+CD8+ T cells (U = 22.00, p = 0.1473), and CD3+CD4+ T cells (t = 3.035, p = 0.0079). Student’s unpaired t test was used.(I) Loss of NKp46-YFP+ cells from peripheral blood in anti-NK1.1-antibody-treated mice. Student’s unpaired t test, t = 15.51, ∗∗∗p = 0.0001.(J) Wild-type mice received either anti-NK1.1 to deplete NK cells or isotype control antibody followed by partial crush of the sciatic nerve on day 0. All mice were treated with IL-2 antibody complex (arrows). Two-way ANOVA. Effect of antibody depletion: F(1,240) = 21.21, p < 0.0001. Bonferroni post-test, ∗∗∗p < 0.001 (t = 4.542).(K) Heatmap showing mean sensitivity to pinprick along the lateral hind paw. Note the broad loss of sensitivity at day 6 in isotype control mice.(L) Area under the curve measurements in isotype control and anti-NK1.1-treated mice following IL-2 complex treatment. days 1–4: t = 0.1508, p = 0.8815; days 5–10: t = 2.390, p = 0.0295; days 11–15: t = 1.040, p = 0.3130). Student’s unpaired t test was used.See also Figure S5. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30712871), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse NKp46/NCR1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse NKp46/NCR1 by Immunocytochemistry/Immunofluorescence Acutely Cultured Embryonic but Not Adult DRG Neurons Reveal Susceptibility to NK-Mediated Cytotoxicity by RAE1(A) Immunolabeling of co-culture (4 h) between embryonic (top) or adult (bottom) DRG neurons ( beta -tubulin, magenta) and either freshly isolated (control) or IL-2-stimulated natural killer (NK) cells (NKp46, green). The inset shows a high-magnification image of NK cell in contact with embryonic DRG neurite.(B) LDH-release cytotoxicity assay of acutely cultured (1 day in vitro) embryonic (top) and adult (bottom) DRG at various Effector (NK):Target (DRG) (E:T) ratios. Matched two-way ANOVA: embryonic DRG, F(1,10) = 100.01, p < 0.0001); adult DRG, F(1,10) = 1.25, p = 0.2982). Three replicate co-cultures for each DRG group.(C) Still images of in vitro time-lapse confocal Ca2+ imaging of rhodamine 3 AM-loaded embryonic (top) and adult (bottom) DRG (magenta) co-cultured with IL-2-stimulated NK cells (green) isolated from adult male NKp46-YFP mice.(D) Frequency histogram (30 s time bins) of neurite Ca2+ events in embryonic (top) and adult (bottom) DRG during NK co-culture. Cumulative area under the curve (right). Student’s paired t test; t = 2.290, p = 0.045. n = 6 fields of view from two repeat co-cultures per group.(E) RT-PCR of mRNA transcripts in freshly isolated splenic NK cells and embryonic and adult DRG.(F) qRT-PCR shows higher Raet1 mRNA expression in embryonic compared to adult DRG tissue. Student’s paired t test; t = 16.16, p < 0.0001. n = 5 mice, or replicates per group.(G) Western blot of embryonic and adult mouse DRG tissue (40 μg loading) with pan-RAE1 antibody and beta -actin control. Images are representative of three independent experiments.(H) Selective siRNA knockdown reduces RAE1 protein (top) and Raet1 mRNA (bottom) expression in embryonic DRG (2 d culture). Student’s unpaired t test; t = 9.060, p = 0.0008. n = 3 mice, or replicates per group.(I) LDH-release cytotoxicity assay of negative control or Raet1-selective siRNA knockdown embryonic DRG. Three replicate co-cultures for each siRNA group. Matched two-way ANOVA F(1,10) = 133.85, p < 0.0001).See also Figure S1. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30712871), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse Mouse NKp46/NCR1 Antibody by Immunohistochemistry View Larger

Detection of Mouse Mouse NKp46/NCR1 Antibody by Immunohistochemistry Peripheral Nerve Injury Regulates Raet1 Expression and Injured Sensory Neurons Show Increased Neurite Fragmentation by Stimulated NK Cells. (H) Sciatic nerve tissue sections from adult male NKp46-YFP mice 7 days after L5 spinal nerve transection injury immunolabeled with anti-GFP (NKp46, green). Arrows indicate NK cells in sciatic nerve. Image collected and cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S0092867418316362), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).

References
  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  3. Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
  4. Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
  5. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  6. Arnon, T. et al. (2004) Blood 103:664.
  7. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  8. Mandelboim, O. et al. (2001) Nature 409:1055.
Entrez Gene IDs
9437 (Human); 17086 (Mouse); 100141419 (Porcine); 102115479 (Cynomolgus Monkey)
Alternate Names
CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

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Citations for Mouse NKp46/NCR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

65 Citations: Showing 1 - 10
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  1. Major Histocompatibility Complex-I Expression on Embryonic Stem Cell-Derived Vascular Progenitor Cells Is Critical for Syngeneic Transplant Survival
    Authors: Mingchao Ma, Shunli Ding, Andreas Lundqvist, Hong San, Fang Fang, Mikhail Konoplyannikov et al.
    Stem Cells
  2. The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression
    Authors: Wenjing Su, Hyun Ho Han, Yan Wang, Boyu Zhang, Bing Zhou, Yuanming Cheng et al.
    Cancer Cell
  3. RNF31 inhibition sensitizes tumors to bystander killing by innate and adaptive immune cells
    Authors: Zhengkui Zhang, Xiangjun Kong, Maarten A. Ligtenberg, Susan E. van Hal-van Veen, Nils L. Visser, Beaunelle de Bruijn et al.
    Cell Reports Medicine
  4. Asbestos accelerates disease onset in a genetic model of malignant pleural mesothelioma
    Authors: Pooyeh Farahmand, Katarina Gyuraszova, Claire Rooney, Ximena L. Raffo-Iraolagoitia, Geeshath Jayasekera, Ann Hedley et al.
    Frontiers in Toxicology
  5. Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids
    Authors: Nikos Koundouros, Evdoxia Karali, Aurelien Tripp, Adamo Valle, Paolo Inglese, Nicholas J.S. Perry et al.
    Cell
  6. Bone-Marrow-Resident NK Cells Prime Monocytes for Regulatory Function during Infection
    Authors: Michael H. Askenase, Seong-Ji Han, Allyson L. Byrd, Denise Morais Morais da Fonseca, Nicolas Bouladoux, Christoph Wilhelm et al.
    Immunity
  7. EZH2 inhibition remodels the inflammatory senescence-associated secretory phenotype to potentiate pancreatic cancer immune surveillance
    Authors: Loretah Chibaya, Katherine C. Murphy, Kelly D. DeMarco, Sneha Gopalan, Haibo Liu, Chaitanya N. Parikh et al.
    Nature Cancer
  8. Cytotoxic lymphocytes target characteristic biophysical vulnerabilities in cancer
    Authors: Maria Tello-Lafoz, Katja Srpan, Elisa E. Sanchez, Jing Hu, Jan Remsik, Yevgeniy Romin et al.
    Immunity
  9. Cytotoxic granzyme C–expressing ILC1s contribute to antitumor immunity and neonatal autoimmunity
    Authors: Briana G. Nixon, Chun Chou, Chirag Krishna, Saïda Dadi, Adam O. Michel, Andrew E. Cornish et al.
    Science Immunology
  10. Senescence-Induced Vascular Remodeling Creates Therapeutic Vulnerabilities in Pancreas Cancer
    Authors: Marcus Ruscetti, John P. Morris, Riccardo Mezzadra, James Russell, Josef Leibold, Paul B. Romesser et al.
    Cell
  11. Anti-tumor necrosis factor-alpha monoclonal antibody suppresses colorectal cancer growth in an orthotopic transplant mouse model
    Authors: T Takasago, R Hayashi, Y Ueno, M Ariyoshi, K Onishi, K Yamashita, Y Hiyama, H Takigawa, R Yuge, Y Urabe, S Oka, Y Kitadai, S Tanaka
    PLoS ONE, 2023-03-30;18(3):e0283822.
  12. KLF2 determines the susceptibility of T cells to immunoregulatory NK cells
    Authors: Waggoner, S;Cox, A;Canaday, L;Katko, A;Feldman, H;Warrick, K;Tselikova, A;Seelamneni, H;Roskin, K;
    Research square
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  13. Amplification of autoimmune organ damage by NKp46-activated ILC1
    Authors: Biniaris-Georgallis, SI;Aschman, T;Stergioula, K;Schreiber, F;Jafari, V;Taranko, A;Karmalkar, T;Kasapi, A;Lenac Rovis, T;Jelencic, V;Bejarano, DA;Fabry, L;Papacharalampous, M;Mattiola, I;Molgora, M;Hou, J;Hublitz, KW;Heinrich, F;Guerra, GM;Durek, P;Patone, G;Lindberg, EL;Maatz, H;Hölsken, O;Krönke, G;Mortha, A;Voll, RE;Clarke, AJ;Hauser, AE;Colonna, M;Thurley, K;Schlitzer, A;Schneider, C;Stamatiades, EG;Mashreghi, MF;Jonjic, S;Hübner, N;Diefenbach, A;Kanda, M;Triantafyllopoulou, A;
    Nature
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  14. Mesothelin Secretion by Pancreatic Cancer Cells Co-opts Macrophages and Promotes Metastasis
    Authors: Luckett, T;Abudula, M;Ireland, L;Glenn, M;Bellomo, G;Stafferton, R;Halloran, C;Ghaneh, P;Jones, R;Schmid, MC;Mielgo, A;
    Cancer research
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Antipodoplanin antibody enhances the antitumor effects of CTLA-4 blockade against malignant mesothelioma by natural killer cells
    Authors: Yoneda, H;Mitsuhashi, A;Yoshida, A;Ogino, H;Itakura, S;Nguyen, NT;Nokihara, H;Sato, S;Shinohara, T;Hanibuchi, M;Abe, S;Kaneko, MK;Kato, Y;Nishioka, Y;
    Cancer science
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  16. Cytotoxic chemotherapy potentiates the immune response and efficacy of combination CXCR4/PD-1 inhibition in models of pancreatic ductal adenocarcinoma
    Authors: Raufi, AG;Pellicciotta, I;Palermo, CF;Sastra, SA;Chen, A;Alouani, E;Maurer, HC;May, M;Iuga, A;Rabadan, R;Olive, KP;Manji, GA;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. An oncolytic virus expressing a full-length antibody enhances antitumor innate immune response to glioblastoma
    Authors: B Xu, L Tian, J Chen, J Wang, R Ma, W Dong, A Li, J Zhang, E Antonio Ch, B Kaur, M Feng, MA Caligiuri, J Yu
    Nature Communications, 2021-10-08;12(1):5908.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition
    Authors: WN Jin, K Shi, W He, JH Sun, L Van Kaer, FD Shi, Q Liu
    Nature Neuroscience, 2020-11-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation
    Authors: FM Kobia, K Preusse, Q Dai, N Weaver, MR Hass, P Chaturvedi, SJ Stein, WS Pear, Z Yuan, RA Kovall, Y Kuang, N Eafergen, D Sprinzak, B Gebelein, EW Brunskill, R Kopan
    PLoS Biol, 2020-10-05;18(10):e3000850.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  20. Corneal dysfunction precedes the onset of hyperglycemia in a mouse model of diet-induced obesity
    Authors: A Hargrave, JA Courson, V Pham, P Landry, S Magadi, P Shankar, S Hanlon, A Das, RE Rumbaut, CW Smith, AR Burns
    PLoS ONE, 2020-09-04;15(9):e0238750.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Antitumor Activity of Amivantamab (JNJ-61186372), an EGFR-cMet Bispecific Antibody, in Diverse Models of EGFR Exon 20 Insertion-Driven NSCLC
    Authors: J Yun, SH Lee, SY Kim, SY Jeong, JH Kim, KH Pyo, CW Park, SG Heo, MR Yun, S Lim, SM Lim, MH Hong, HR Kim, M Thayu, JC Curtin, RE Knoblauch, MV Lorenzi, A Roshak, BC Cho
    Cancer Discov, 2020-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Forced expression of CXCL10 prevents liver metastasis of colon carcinoma cells by the recruitment of natural killer cells
    Authors: N Kikuchi, J Ye, J Hirakawa, H Kawashima
    Biol. Pharm. Bull., 2018-11-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice
    Authors: S Nishina, A Yamauchi, T Kawaguchi, K Kaku, M Goto, K Sasaki, Y Hara, Y Tomiyama, F Kuribayash, T Torimura, K Hino
    Cell Mol Gastroenterol Hepatol, 2018-09-11;7(1):115-134.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization
    Authors: EE Er, M Valiente, K Ganesh, Y Zou, S Agrawal, J Hu, B Griscom, M Rosenblum, A Boire, E Brogi, FG Giancotti, M Schachner, S Malladi, J Massagué
    Nat. Cell Biol., 2018-07-23;20(8):966-978.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Therapeutic Potential of Leelamine, a Novel Inhibitor of Androgen Receptor and Castration-Resistant Prostate Cancer
    Authors: KB Singh, X Ji, SV Singh
    Mol. Cancer Ther., 2018-07-20;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Preclinical, non-genetic models of lung adenocarcinoma: a comparative survey
    Authors: F Janker, W Weder, JH Jang, W Jungraithm
    Oncotarget, 2018-07-17;9(55):30527-30538.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment.
    Authors: A Martin, C Seignez, C Racoeur, N Isambert, Isambert N, N Mabrouk, Mabrouk N, A Scagliarin, Scagliarini A, S Reveneau, Reveneau S, L Arnould, Arnould L, A Bettaieb, JF Jeannin, Jeannin J, C Paul
    Oncotarget, 2018-06-19;9(47):28364-28378.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. NKp46 Calibrates Tumoricidal Potential of Type 1 Innate Lymphocytes by Regulating TRAIL Expression
    Authors: G Turchinovi, S Ganter, A Bärenwaldt, D Finke
    J. Immunol., 2018-04-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. NK cell heparanase controls tumor invasion and immune surveillance
    Authors: EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth
    J. Clin. Invest., 2017-06-05;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  30. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  31. Independent control of natural killer cell responsiveness and homeostasis at steady-state by CD11c+ dendritic cells
    Sci Rep, 2016-12-01;6(0):37996.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  32. Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells
    J Leukoc Biol, 2016-08-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo
    Sci Rep, 2016-08-01;6(0):30636.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  34. Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival
    Authors: W Rabacal, SK Pabbisetty, KL Hoek, D Cendron, Y Guo, D Maseda, E Sebzda
    Proc Natl Acad Sci USA, 2016-04-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  35. IL-15 Superagonist-Mediated Immunotoxicity: Role of NK Cells and IFN-gamma.
    Authors: Guo Y, Luan L, Rabacal W, Bohannon J, Fensterheim B, Hernandez A, Sherwood E
    J Immunol, 2015-07-27;195(5):2353-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
    Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
    J Leukoc Biol, 2015-01-12;97(3):583-98.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC, IHC-P
  37. Antigen dependently activated cluster of differentiation 8-positive T cells cause perforin-mediated neurotoxicity in experimental stroke.
    Authors: Mracsko E, Liesz A, Stojanovic A, Lou W, Osswald M, Zhou W, Karcher S, Winkler F, Martin-Villalba A, Cerwenka A, Veltkamp R
    J Neurosci, 2014-12-10;34(50):16784-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  38. BCMab1, a monoclonal antibody against aberrantly glycosylated integrin alpha3beta1, has potent antitumor activity of bladder cancer in vivo.
    Authors: Li C, Yang Z, Du Y, Tang H, Chen J, Hu D, Fan Z
    Clin Cancer Res, 2014-07-07;20(15):4001-13.
    Species: Mouse
    Sample Types:
  39. Role of CC chemokine receptor 4 in natural killer cell activation during acute cigarette smoke exposure.
    Authors: Stolberg V, Martin B, Mancuso P, Olszewski M, Freeman C, Curtis J, Chensue S
    Am J Pathol, 2013-12-09;184(2):454-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  40. TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Authors: Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke C, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lepinasse F, Marvel J, Verhoeyen E, Cosset F, Peeper D, Smyth M, Londono-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec J, Stoppacciaro A, Leonetti C, Vivier E, Gilson E
    Nat Cell Biol, 2013-06-23;15(7):818-28.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  41. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
    Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL
    PLoS Pathog., 2012-01-12;8(1):e1002481.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  42. Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
    Authors: Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O
    J. Immunol., 2011-08-17;187(6):3096-103.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  43. Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells.
    Authors: Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B
    Proc. Natl. Acad. Sci. U.S.A., 2010-05-10;107(21):9759-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  44. Distinct phenotype and function of NK cells in the pancreas of nonobese diabetic mice.
    Authors: Brauner H, Elemans M, Lemos S, Broberger C, Holmberg D, Flodstrom-Tullberg M, Karre K, Hoglund P
    J. Immunol., 2010-02-03;184(5):2272-80.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC
  45. T-cell recruitment and Th1 polarization in adipose tissue during diet-induced obesity in C57BL/6 mice.
    Authors: Strissel KJ, DeFuria J, Shaul ME, Bennett G, Greenberg AS, Obin MS
    Obesity (Silver Spring), 2010-01-28;18(10):1918-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  46. Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.
    Authors: Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M
    J. Immunol., 2009-10-19;183(10):6489-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  47. Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells.
    Authors: Kang SJ, Liang HE, Reizis B, Locksley RM
    Immunity, 2008-11-14;29(5):819-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  48. CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells.
    Authors: Vosshenrich CA, Lesjean-Pottier S, Hasan M, Richard-Le Goff O, Corcuff E, Mandelboim O, Di Santo JP
    J. Exp. Med., 2007-10-08;204(11):2569-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  49. Natural Killer Cells Degenerate Intact Sensory Afferents following Nerve Injury
    Authors: Davies AJ, Kim HW, Gonzalez-Cano R et al.
    Cell
  50. Blockade of Stromal Gas6 Alters Cancer Cell Plasticity, Activates NK Cells, and Inhibits Pancreatic Cancer Metastasis
    Authors: Lucy Ireland, Teifion Luckett, Michael C. Schmid, Ainhoa Mielgo
    Frontiers in Immunology
  51. Moderate Exercise Inhibits Age-Related Inflammation, Liver Steatosis, Senescence, and Tumorigenesis
    Authors: Bianchi A, Marchetti L, Hall Z, et al.
    Journal of immunology (Baltimore, Md. : 1950)
  52. KLRG1+ NK Cells Protect T-bet–Deficient Mice from Pulmonary Metastatic Colorectal Carcinoma
    Authors: Muriel Malaisé, Jordi Rovira, Philipp Renner, Elke Eggenhofer, Manije Sabet-Baktach, Margareta Lantow et al.
    The Journal of Immunology
  53. A novel Cytochrome P450 26A1 expressing NK cell subset at the mouse maternal‐foetal interface
    Authors: Dan‐Ping Wei, Dan‐Dan Li, Ai‐Qin Gu, Wen‐Heng Ji, Ying Yang, Jing‐Pian Peng
    Journal of Cellular and Molecular Medicine
  54. Natural killer cells activated through NKG2D mediate lung ischemia-reperfusion injury
    Authors: Daniel R. Calabrese, Emily Aminian, Benat Mallavia, Fengchun Liu, Simon J. Cleary, Oscar A. Aguilar et al.
    Journal of Clinical Investigation
  55. Group 3 innate lymphoid cells continuously require the transcription factor GATA-3 after commitment
    Authors: Chao Zhong, Kairong Cui, Christoph Wilhelm, Gangqing Hu, Kairui Mao, Yasmine Belkaid et al.
    Nature Immunology
  56. MYC is a clinically significant driver of mTOR inhibitor resistance in breast cancer
    Authors: Jinhyuk Bhin, Julia Yemelyanenko, Xue Chao, Sjoerd Klarenbeek, Mark Opdam, Yuval Malka et al.
    Journal of Experimental Medicine
  57. DPP inhibition alters the CXCR3 axis and enhances NK and CD8+ T cell infiltration to improve anti-PD1 efficacy in murine models of pancreatic ductal adenocarcinoma
    Authors: Allison A Fitzgerald, Shangzi Wang, Veena Agarwal, Emily F Marcisak, Annie Zuo, Sandra A Jablonski et al.
    Journal for ImmunoTherapy of Cancer
  58. CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cells
    Authors: Abdul S. Qadir, Jean Philippe Guégan, Christophe Ginestier, Assia Chaibi, Alban Bessede, Emmanuelle Charafe-Jauffret et al.
    iScience
  59. Transcriptome analysis following neurotropic virus infection reveals faulty innate immunity and delayed antigen presentation in mice susceptible to virus‐induced demyelination
    Authors: Malgorzata Ciurkiewicz, Stefan Floess, Michael Beckstette, Maren Kummerfeld, Wolfgang Baumgärtner, Jochen Huehn et al.
    Brain Pathology
  60. The atypical receptor CCRL2 is essential for NK cell-dependent resistance against lung cancer
    Authors: Annalisa Del Prete, Francesca Sozio, Tiziana Schioppa, Andrea Ponzetta, William Vermi, Stefano Calza et al.
    Cancer Immunology Research
  61. A point mutation in the Ncr1 signal peptide impairs the development of innate lymphoid cell subsets
    Authors: Francisca F. Almeida, Sara Tognarelli, Antoine Marçais, Andrew J. Kueh, Miriam E. Friede, Yang Liao et al.
    OncoImmunology
  62. In Vivo Measurement of Granzyme Proteolysis from Activated Immune Cells with PET
    Authors: Ning Zhao, Conner Bardine, André Luiz Lourenço, Yung-hua Wang, Yangjie Huang, Simon J. Cleary et al.
    ACS Central Science
  63. Enhanced Sensitivity of Colon Tumor Cells to Natural Killer Cell Cytotoxicity after Mild Thermal Stress is regulated through Heat Shock Factor 1 mediated Expression of MICA
    Authors: Baris E. Dayanc, Sanjay Bansal, Ali Osmay Gure, Sandra O. Gollnick, Elizabeth A. Repasky
    International Journal of Hyperthermia
  64. Metabolic diversity within breast cancer brain-tropic cells determines metastatic fitness
    Authors: Parida PK, Marquez-Palencia M, Nair V et al.
    Cell metabolism
  65. Ras/MAPK signalling intensity defines subclonal fitness in a mouse model of hepatocellular carcinoma
    Authors: Anthony Lozano, Francois-Régis Souche, Carine Chavey, Valérie Dardalhon, Christel Ramirez, Serena Vegna et al.
    eLife

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Mouse NKp46/NCR1 Antibody
By Efstathios Stamatiades on 07/10/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Salivary gland tissue Species: Mouse

PLP-fixed salivary gland cryosections were stained with anti NKp46/NCR1 for 4h at room temperature. After washing, the samples were stained with Donkey anti-goat alexa546 2ndary antibody for 1h at room temperature. NKp46 in red, CD3 in green & Hoechst in blue.

Immunocytochemistry/Immunofluorescence Mouse NKp46/NCR1 Antibody AF2225