Mouse Nodal Antibody

Catalog #: MAB1315
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1315
MAB1315-SP

All Nodal Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Nodal Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Nodal in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 25 ‑ 50% cross‑reactivity with recombinant Drosophila (rd) Dpp and no cross-reactivity with rmArtemin, rmCripto, rhGDNF, rhLAP, rhLatent TGF‑ beta 1, rhLefty‑A, rmLefty‑1, rrMIS, rhNeurturin, rhTGF‑ alpha, rhTGF‑ beta 1, rhTGF‑ beta 1.2, rhTGF‑ beta 2, rhTGF‑ beta 3, or recombinant amphibian TGF‑ beta 5.

Source

Monoclonal Rat IgG₁ Clone # 209009

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Nodal
His245-Leu354
Accession # P43021

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Nodal (Catalog # 1315-ND)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nodal

Nodal is a secreted protein that is a member of the Transforming Growth Factor-beta (TGF-beta ) superfamily. Nodal was named for its localized expression in the mouse node during gastrulation, and is first detected in the early primitive streak during mesoderm formation. Expression of the Nodal gene occurs asymmetrically in the left, but not right, lateral plate during somitogenesis. Nodal proteins play crucial roles in mesoderm formation and both anterior-posterior and left-right axis formation during vertebrate development. Members of the Nodal gene family include mouse Nodal, chick cNR-1, frog Xnr1-4, and zebrafish cyclops. Biologically active Nodal is a disulfide-linked homodimer and contains all seven of the cysteine residues necessary for formation of the “cysteine knot” characteristic of TGF-beta -related molecules. Mouse Nodal is 34 - 39% homologous in the conserved region to other TGF-beta superfamily members. Nodal has been shown to signal through a mechanism related to the Activin pathway, and signaling is mediated through both Smad2 and 3. Nodal signaling utilizes type II Activin receptors, together with ALK4/ActRIB, or the orphan type I receptor ALK7. Nodal interacts extracellularly with members of other protein families, including Cerberus, Lefty, and EGF-CFC ligands, such as Cripto. While the Cerberus and Lefty families act as Nodal antagonists, the EGF-CFC molecules act as co-receptors to facilitate Nodal signaling. The resulting concert of regulated Nodal activity allows for the precise control of mesoderm formation, neural patterning, and axis positioning and patterning during early vertebrate development.

References

Kumar, A. et al. (2001) J. Biol. Chem. 276:656.
Reissmann, E. et al. (2001) Genes & Dev. 15: 2010.
Schier, A. and M. Shen (1999) Nature 403:385.
Zhou, X. et al. (1993) Nature 361:543.

Entrez Gene IDs

4838 (Human); 18119 (Mouse)

Alternate Names

BMP-16; MGC138230; nodal homolog (mouse); nodal homolog; Nodal; nodal, mouse, homolog

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Early Mesodermal Lineage Markers

Citation for Mouse Nodal Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Expression of nodal signalling components in cycling human endometrium and in endometrial cancer.
Authors: Papageorgiou I, Nicholls PK, Wang F
Reprod. Biol. Endocrinol., 2009-10-29;7(0):122.
Species: Human
Sample Types: Uterine Lavage Fluid
Applications: Western Blot