Mouse Opticin Antibody

Catalog #: AF3547
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3547
AF3547-SP

All Opticin Antibodies

Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Opticin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Opticin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human Opticin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Opticin
Ala20-Thr328 (Tyr168Asp)
Accession # Q920A0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Opticin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Opticin

Opticin, also known as Oculoglycan, is an extracellular matrix protein that belongs to the class III subfamily of the small leucine-rich repeat (SLRP) proteoglycan family. It was initially discovered as a protein associated with vitreous humor collagen fibrils and has since been localized also to numerous ocular tissues and fetal liver. Mouse and human Opticin share 73% amino acid sequence identity.

Entrez Gene IDs

26254 (Human); 269120 (Mouse)

Alternate Names

oculoglycan; Oculogylcan; OPT; OPTC; Opticin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Opticin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression
Authors: J Pang, L Le, Y Zhou, R Tu, Q Hou, D Tsuchiya, N Thomas, Y Wang, Z Yu, R Alexander, M Thexton, B Lewis, T Corbin, M Durnin, H Li, R Ashery-Pad, D Yan, T Xie
Cell Reports, 2021-01-12;34(2):108603.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC
Dominant inhibition of lens placode formation in mice.
Authors: Zhang Y, Burgess D, Overbeek PA, Govindarajan V
Dev. Biol., 2008-08-26;323(1):53-63.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
PEDF Expression Affects the Oxidative and Inflammatory State of Choroidal Endothelial Cells
Authors: M Farnoodian, CM Sorenson, N Sheibani
Am. J. Physiol., Cell Physiol., 2018-01-10;0(0):.