Mouse OSMR beta Biotinylated Antibody

Catalog #: BAM662 Datasheet / COA / SDS

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BAM662

All OSMR beta Antibodies

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Mouse OSMR beta Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse OSM R beta.

Source

Monoclonal Rat IgG_2A Clone # 118125

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse OSM R beta
Glu24-Leu738
Accession # O70458

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed frozen sections of mouse embryo (E13.5-15.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OSMR beta

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the Leukemia Inhibitory Factor Receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM Receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) residue type I transmembrane protein which contains a 23 aa residue signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity, but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.

References

Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
Tanaka, M. et al. (1999) Blood 93:804.

Long Name

Oncostatin M Receptor beta

Entrez Gene IDs

9180 (Human); 18414 (Mouse); 310132 (Rat)

Alternate Names

IL-31 receptor subunit beta; IL-31R subunit beta; IL-31RB; IL-31R-beta; Interleukin-31 receptor subunit beta; MGC150627; MGC75127; oncostatin M receptor; oncostatin-M specific receptor beta subunit; oncostatin-M-specific receptor subunit beta; OSM R beta; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1