Mouse OX40/TNFRSF4 Antibody

Catalog #: AF1256
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1256
AF1256-SP

All OX40 Antibodies

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Citations (2)

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Mouse OX40/TNFRSF4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse OX40/TNFRSF4 in direct ELISAs and Western blots. In these formats, less than 2% cross-reactivity with recombinant mouse (rm) EDAR, rm4-BB, rmCD27, rmDR3, rmGITR, rmNGF R, rmCD30, rmCD40, rmFas, rmOPG, rmRANK, rmTNF RI, and rmTNF RII is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse OX40/TNFRSF4
Val20-Pro211
Accession # P47741

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse OX40/TNFRSF4 Fc Chimera (Catalog # 1256-OX)

Agonist Activity

Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3 [Saoulli, K. et al. (1998) J. Exp. Med. 187(11):1849; Cannons, J. et al. (2001) J. Immunol. 167:1313.].
The ED₅₀ for this effect is typically 0.4 - 1.2 μg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OX40

OX40, also known as CD134, was originally identified as an activated rat CD4⁺ T cell-surface antigen that is recognized by the monoclonal antibody MRC OX40. It is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF4. Mouse OX40 cDNA encodes a 256 amino acid (aa) residues type I transmembrane precursor protein with a putative 19 aa signal peptide, a 192 aa extracellular domain containing 4 TNFR-cysteine rich repeats, a 25 aa transmembrane domain and a 36 aa cytoplasmic region. A naturally occurring soluble OX40 has also been identified in human serum. Mouse OX40 shares approximately 63% and 90% aa sequence identity with its human and rat counterparts, respectively. OX40 is a T cell activation antigen that is expressed primarily on activated CD4⁺ T cells, but is also expressed on activated human and mouse CD8⁺T cells. The ligand of OX40 is OX40 ligand (OX40L), also known as gp34, a type II transmembrane glycoprotein belonging to the TNF superfamily. OX40L is expressed on activated B cells, T cells, dendritic cells and endothelial cells. Ligation of OX40 on T cells by OX40L or an agonistic antibody can promote clonal expansion, long-term T cell survival, and enhance memory T cell development. In vivo, blockade of OX40/OX40L interaction has been useful for treating autoimmune disease and graft-versus-host disease in animal models. Activation of OX40 has also been utilized to enhance the potency of vaccines and augment anti-tumor immunity (1-9).

References

Paterson, D.J. et al. (1987) Mol. Immunol. 24:1281.
Mallett, S. et al. (1990) EMBO J. 9:1063.
Calderhead, D.M. et al. (1993) J. Immunol. 151:5261.
Baum, P.R. et al. (1994) EMBO J. 13:3992.
Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
Bansal-Pakala, P. et al. (2001) Nature Medicine 7:907.
Rogers, P.R. et al. (2001) Immunity 15:445.
Taylor, L. and H.J. Schwarz (2001) Immunol. Methods 255:67.
Weinberg, A.D. (2002) Trends in Immunol. 23:102.

Entrez Gene IDs

7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)

Alternate Names

ACT-135; ACT35 antigen; ACT35ATC35 antigen; CD134 antigen; CD134; Ly-70; OX40 cell surface antigen; OX40 homologue; OX40; OX40L receptor; OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; TNFRSF4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen

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Citations for Mouse OX40/TNFRSF4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Type 2 innate lymphoid cells drive CD4+ Th2 cell responses.
Authors: Mirchandani, Ananda S, Besnard, Anne-Gae, Yip, Edwin, Scott, Charlott, Bain, Calum C, Cerovic, Vuk, Salmond, Robert J, Liew, Foo Y
J Immunol, 2014-01-27;192(5):2442-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
OX40 complexes with phosphoinositide 3-kinase and protein kinase B (PKB) to augment TCR-dependent PKB signaling.
Authors: So T, Choi H, Croft M
J. Immunol., 2011-02-02;186(6):3547-55.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot