Mouse Patched 2/PTCH2 Antibody

Catalog #: MAB8096 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8096
MAB8096-SP

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Mouse Patched 2/PTCH2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Patched 2/PTCH2 in ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Patched 1 or recombinant human Patched 2 is observed.

Source

Monoclonal Rat IgG_2A Clone # 865619

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Patched 2/PTCH2
Glu79-Ser394
Accession # O35595

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Patched 2/PTCH2 in Mouse Testis. Patched 2/PTCH2 was detected in immersion fixed frozen sections of mouse testis using Rat Anti-Mouse Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB8096) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Patched 2/PTCH2

Patched 2 (Ptch2) is a 180-200 kDa 12 transmembrane member of the Patched family. It is less widely expressed than Patched 1, but is found at high levels in skin and spermatocytes. The 1182 amino acid (aa) mouse Patched 2 contains two terminal cytoplasmic domains between aa 1-57 and aa 1108-1182. Together with Smoothened (SMO), Patched 2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (Shh). Over the first extracellular loop (aa 79-394), mouse Patched 2 shares 95% aa sequence identity with human Patched 2. Mouse Patched 2 has one potential 1117 aa isoform that lacks aa 207-271.

Entrez Gene IDs

8643 (Human); 19207 (Mouse)

Alternate Names

patched (Drosophila) homolog 2; Patched 2; patched homolog 2 (Drosophila); patched homolog 2; protein patched homolog 2; ptc2; PTCH2