Mouse PGLYRP2/PGRP-L Antibody

Catalog #: MAB4704 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4704
MAB4704-SP

Detection of PGLYRP2/PGRP-L in Mouse Liver Cells by Flow Cytometry.

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Mouse PGLYRP2/PGRP-L Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PGLYRP2/PGRP-L in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) PGRP-S and rmPGRP-1 beta is observed.

Source

Monoclonal Rat IgG_2A Clone # 439728

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PGLYRP2/PGRP-L
Ser23-Asn530
Accession # Q8VCS0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse PGLYRP2/PGRP-L

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of PGLYRP2/PGRP-L antibody in Mouse Liver Cells antibody by Flow Cytometry.

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Detection of PGLYRP2/PGRP-L in Mouse Liver Cells by Flow Cytometry. Mouse liver single-cell suspension was stained with Rat Anti-Mouse PGLYRP2/PGRP-L Monoclonal Antibody (Catalog # MAB4704, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGLYRP2/PGRP-L

PGRP-L (peptidoglycan recognition protein‑long; also called PGRP-2 or TagL) is a 530 amino acid (aa) member of the PGRP family that is expressed in the liver. All mouse PGRPs bind peptidoglycan and Gram-positive bacteria, and may function as innate immunity pattern recognition molecules. PGRP-L is a Zn²⁺-dependent N‑acetylmuramoyl-L-alanine amidase that hydrolyzes cell wall glycopeptides. It contains two membrane-spanning segments, with both termini thought to be extracellular, but is also reported in serum. A truncated 450 aa isoform has been reported. Overall aa identity between mouse and human PGRP-L is 68%.

Long Name

Peptidoglycan Recognition Protein Long/Peptidoglycan Recognition Protein 2

Entrez Gene IDs

114770 (Human); 57757 (Mouse)

Alternate Names

EC 3.5.1.28; peptidoglycan recognition protein 2tagl-beta; peptidoglycan recognition protein L; PGLYRP2; PGLYRPL; PGLYRPLHMFT0141; PGRP2; PGRPL; PGRP-L; PGRP-LN-acetylmuramoyl-L-alanine amidase; PGRPLPeptidoglycan recognition protein long; TAGL; tagL-alpha; TAGL-like