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Mouse pIgR Antibody

Catalog #: AF2800
22 Citations Datasheet / COA / SDS
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AF2800
AF2800-SP
Detection of Mouse pIgR by Western Blot.
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Citations (22)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse pIgR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse pIgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse pIgR
Lys19-Lys645
Accession # O70570
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of mouse IgM to immobilized Recombinant Mouse pIgR (Catalog # 2800-PG) coated at 5 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse pIgR antibody by Western Blot. View Larger

Detection of Mouse pIgR by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for pIgR at approximately 85 to 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry pIgR antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

pIgR in Mouse Liver. pIgR was detected in immersion fixed frozen sections of mouse liver using Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm and plasma membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: pIgR

The mouse polymeric immunoglobulin receptor (plgR; also known as membrane secretory component) is a 115 kDa type I transmembrane glycoprotein that is synthesized as a 771 amino acid (aa) precursor. It includes an 18 aa signal sequence, a 627 aa extracellular domain (ECD) (aa 19‑645), a 23 aa transmembrane segment (aa 646‑668), and a 143 aa cytoplasmic region (aa 669‑771) (1‑3). The ECD consists of five V-type Ig-like domains and a sixth non-Ig domain that connects to the transmembrane region. The ECD of mouse plgR is 65%, 69%, 85%, 62% and 62% aa identical to the equivalent region in human, porcine, rat, bovine and canine, respectively. plgR is expressed on secretory epithelial cells and serves as a carrier that transports IgA and IgM across epithelium (1, 2, 4). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via domains #1 and #5 of the plgR. A rearrangement then occurs where a disulfide bond forms between domain #5 of the plgR and an IgA heavy chain (2). This complex is then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is generated by proteolytic cleavage of the complex in the sixth extracellular domain of plgR and released into the lumen (5). This proteolytically generated plgR fragment is referred to as secretory component (SC). Notably, in human, plgR transcytoses constitutively, with or without ligand, creating both a bound and free, 78 kDa SC following cleavage (3). In mouse, this event would be expected to generate a 95 kDa fragment (1). The receptor component of the complex anchors the SIgA molecule to mucous (6), where it serves to protect mucous membranes that form a barrier between the interior of the body and the external environment (7).

References
  1. de Groot, N. et al. (1999) Transgenic Res. 8:125.
  2. Piskurich, J. et al. (1995) J. Immunol. 154:1735.
  3. Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
  4. Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
  5. Asano, M. et al. (2004) Immunology 112:583.
  6. Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
  7. Uren, T. et al. (2003) J. Immunol. 170:2531.
Long Name
Polymeric Immunoglobulin Receptor
Entrez Gene IDs
5284 (Human); 18703 (Mouse)
Alternate Names
FLJ22667; hepatocellular carcinoma associated protein TB6; Hepatocellular carcinoma-associated protein TB6; MGC125361; MGC125362; pIgR; Poly-Ig receptor; polymeric immunoglobulin receptor

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Citations for Mouse pIgR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Intestinal Permeability and IgA Provoke Immune Vasculitis Linked to Cardiovascular Inflammation
    Authors: Noval Rivas M, Wakita D, Franklin MK et al.
    Immunity
  2. Porcine Milk-Derived Small Extracellular Vesicles Promote Intestinal Immunoglobulin Production through pIgR
    Authors: Bin Zeng, Hailong Wang, Junyi Luo, Meiying Xie, Zhengjiang Zhao, Xingping Chen et al.
    Animals (Basel)
  3. Intestinal epithelium dysfunctions cause IgA deposition in the kidney glomeruli of intestine-specific Ap1m2-deficient mice
    Authors: Kinashi, Y;Tanaka, K;Kimura, S;Hirota, M;Komiyama, S;Shindo, T;Hashiguchi, A;Takahashi, D;Shibata, S;Karaki, SI;Ohno, H;Hase, K;
    EBioMedicine
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  4. Single-cell spatial transcriptomics reveals a dynamic control of metabolic zonation and liver regeneration by endothelial cell Wnt2 and Wnt9b
    Authors: Shikai Hu, Silvia Liu, Yu Bian, Minakshi Poddar, Sucha Singh, Catherine Cao et al.
    Cell Reports Medicine
  5. Lung immunoglobulin A immunity dysregulation in cystic fibrosis
    Authors: AM Collin, M Lecocq, S Noel, B Detry, FM Carlier, F Aboubakar, C Bouzin, T Leal, M Vermeersch, V De Rose, L Regard, C Martin, PR Burgel, D Hoton, S Verleden, A Froidure, C Pilette, S Gohy
    EBioMedicine, 2020-09-11;60(0):102974.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein
    Authors: B Zeng, D Wang, H Wang, T Chen, J Luo, Q Xi, J Sun, Y Zhang
    Nutrients, 2020-09-08;12(9):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  7. Fecal IgA Levels Are Determined by Strain-Level Differences in Bacteroides ovatus and Are Modifiable by Gut Microbiota Manipulation
    Authors: Chao Yang, Ilaria Mogno, Eduardo J. Contijoch, Joshua N. Borgerding, Varun Aggarwala, Zhihua Li et al.
    Cell Host & Microbe
  8. Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells
    Authors: C Bornstein, S Nevo, A Giladi, N Kadouri, M Pouzolles, F Gerbe, E David, A Machado, A Chuprin, B Tóth, O Goldberg, S Itzkovitz, N Taylor, P Jay, VS Zimmermann, J Abramson, I Amit
    Nature, 2018-07-18;559(7715):622-626.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation
    Authors: M Basic, M Buettner, LM Keubler, A Smoczek, I Bruesch, S Buchheiste, A Bleich
    Sci Rep, 2018-01-15;8(1):719.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces
    Authors: KM Azzam, JH Madenspach, DW Cain, L Lai, KM Gowdy, P Rai, K Janardhan, N Clayton, W Cunningham, H Jensen, PS Patel, JF Kearney, GA Taylor, MB Fessler
    JCI Insight, 2017-08-17;2(16):.
    Species: Mouse
    Sample Types: BALF, Tissue Homogenates
    Applications: Western Blot
  11. Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA
    Authors: K Nakanishi, S Morikane, S Ichikawa, K Kurohane, Y Niwa, Y Akimoto, S Matsubara, H Kawakami, H Kobayashi, Y Imai
    Sci Rep, 2017-04-03;7(0):45843.
    Species: Plant
    Sample Types: Crude Leaf Extracts, Whole Tissue
    Applications: ELISA Development (Capture), IHC
  12. Contribution of mucus concentration and secreted mucins Muc5ac and Muc5b to the pathogenesis of muco-obstructive lung disease
    Authors: Alessandra Livraghi-Butrico, Barbara R. Grubb, Kristen J. Wilkinson, Allison S. Volmer, Kimberly A. Burns, Christopher M. Evans et al.
    Mucosal Immunology
  13. Exploitation of the Polymeric Immunoglobulin Receptor for Antibody Targeting to Renal Cyst Lumens in Polycystic Kidney Disease.
    Authors: Olsan E, Matsushita T, Rezaei M, Weimbs T
    J Biol Chem, 2015-04-28;290(25):15679-86.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variation.
    Authors: Moon C, Baldridge M, Wallace M, Burnham C, Virgin H, Stappenbeck T
    Nature, 2015-02-16;521(7550):90-3.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  15. Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferation.
    Authors: Hegab, Ahmed E, Ha, Vi Luan, Bisht, Bharti, Darmawan, Daphne O, Ooi, Aik T, Zhang, Kelvin X, Paul, Manash K, Kim, Yeon Sun, Gilbert, Jennifer, Attiga, Yasser S, Alva-Ornelas, Jackelyn, Nickerson, Derek W, Gomperts, Brigitte
    Stem Cells Dev, 2014-01-02;23(6):664-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. IL-25 Improves IgA Levels During Parenteral Nutrition Through the JAK-STAT Pathway
    Authors: Aaron F. Heneghan, Joseph F. Pierre, Kenneth A. Kudsk
    Annals of Surgery
  17. Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis.
    Authors: Moon C, VanDussen K, Miyoshi H, Stappenbeck T
    Mucosal Immunol, 2013-11-13;7(4):818-28.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012-07-10;7(0):87-104.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  19. Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides.
    Authors: Frantz A, Rogier E, Weber C, Shen L, Cohen D, Fenton L, Bruno M, Kaetzel C
    Mucosal Immunol, 2012-04-11;5(5):501-12.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid.
    Authors: Kang X, Li N, Wang M, Boontheung P, Sioutas C, Harkema JR, Bramble LA, Nel AE, Loo JA
    Proteomics, 2010-02-01;10(3):520-31.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  21. Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens.
    Authors: Shim DH, Ko HJ, Volker G, Potter AA, Mutwiri G, Babiuk LA, Kweon MN
    Vaccine, 2010-01-08;28(11):2311-7.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  22. The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant.
    Authors: Libanova R, Ebensen T, Schulze K, Bruhn D, Norder M, Yevsa T, Morr M, Guzman CA
    Vaccine, 2010-01-07;28(10):2249-58.
    Species: Mouse
    Sample Types: Mucosal Lavage Fluid
    Applications: ELISA Development

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