Mouse PILR-beta Antibody

Catalog #: AF3525 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3525
AF3525-SP

All PILR-beta Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PILR-beta Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PILR-beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse PILR-alpha is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PILR-beta
Ala28-Gly193
Accession # Q2YFS2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse PILR-beta (Catalog # 3525-PR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PILR-beta

PILR-beta (paired immunoglobulin-like type 2 receptor-beta) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-alpha and it likely arose through PILR-alpha gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-beta and -alpha should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-beta and -alpha show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-beta is a 196 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a short 8 aa cytoplasmic region. The ECD shows a V-type Ig-like domain (aa 39‑135), while the TM segment contains a positively-charged Lys at position # 202. This Lys is known to interact with the transmembrane signaling adaptor protein DAP12, making PILR-beta an activating receptor. Activation of PILR-beta through CD99 ligation induces NK cell cytotoxicity and dendritic cell secretion of NO and TNF-alpha (3). Mouse PILR-beta is found on NK cells, neutrophils, macrophages, and monocyte-derived dendritic cells (3). Mouse PILR-beta ECD is 44% and 75% aa identical to human and rat PILR-beta ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-alpha (3). Evidence suggests that mouse PILR-beta will not be active in a human system (3). Potential isoforms of PILR-beta have been reported. One shows an alternate start site at Met14, a second shows a 21 aa substitution for the C-terminal 67 aa, and a third exhibits multiple polymorphisms for an overall aa identity of 86% (4, 5, 6).

References

Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.
Lanier, L.L. (2001) Curr. Opin. Immunol. 13:326.
Shiratori, I. et al. (2004) J. Exp. Med. 199:525.
Genbank Accession # NP_573472.
Genbank Accession # BAC29442.
Genbank Accession # XP_001480583.

Long Name

Paired-Ig-likeType 2 Receptor beta

Entrez Gene IDs

29990 (Human); 170741 (Mouse)

Alternate Names

activating receptor PILRbeta; Activating receptor PILR-beta; Cell surface receptor FDFACT; cell surface receptor FDFACT1; cell surface receptor FDFACT2; FDFACT; FDFACT1; FDFACT2; paired immunoglobin-like receptor beta; paired immunoglobin-like type 2 receptor beta; paired immunoglobulin-like receptor beta; paired immunoglobulin-like type 2 receptor beta; PILRB; PILRbeta; PILR-beta