Mouse Podoplanin Biotinylated Antibody

Catalog #: BAF3244
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF3244

All Podoplanin Antibodies

Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Podoplanin Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Podoplanin in Western blots. In Western blots, approximately 1% cross-reactivity with recombinant human Podoplanin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin
Gln21-Lys133
Accession # Q62011

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Podoplanin Fc Chimera (Catalog # 3244-PL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podoplanin

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Long Name

Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A

Entrez Gene IDs

10630 (Human); 14726 (Mouse); 54320 (Rat)

Alternate Names

36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha

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Product Datasheet

COA

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Related Research Areas

Citations for Mouse Podoplanin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Podoplanin neutralization improves cardiac remodeling and function after acute myocardial infarction
Authors: Maria Cimini, Venkata Naga Srikanth Garikipati, Claudio de Lucia, Zhongjian Cheng, Chunlin Wang, May M. Truongcao et al.
JCI Insight
Single-cell RNA sequencing identifies response of renal lymphatic endothelial cells to acute kidney injury
Authors: Creed, HA;Kannan, S;Tate, BL;Banerjee, P;Mitchell, BM;Chakraborty, S;Rutkowski, JM;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Selection
Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells
Authors: MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields
Nat Commun, 2018-03-05;9(1):948.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC