Mouse Prostasin/Prss8 Antibody

Catalog #: MAB2968 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2968
MAB2968-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Prostasin/Prss8 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Prostasin/Prss8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Prostasin is observed.

Source

Monoclonal Rat IgG_2A Clone # 430814

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Prostasin/Prss8
Ala30-Gln289
Accession # NP_579929

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Prostasin/Prss8 (Catalog # 2968-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prostasin/Prss8

Prostasin encoded by the Prss8 gene, also known as channel activating protease 1, is a serine protease with a trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of prostasin is involved in the regulation of epithelial sodium channels (4, 5). The enzymatic activity of Prostasin is effectively inhibited by HAI-1 and HAI-2B (Catalog # 1141-PI and 1107-PI).

References