Home / Rae-1 / Mouse Rae-1 Pan Specific Fluorescein-conjugated Antibody

Mouse Rae-1 Pan Specific Fluorescein-conjugated Antibody

Catalog #: FAB17582F
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB17582F
Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Rae-1 Pan Specific Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1. It recognizes Rae-1 alpha, beta, δ, gamma and epsilon.
Source
Monoclonal Rat IgG2A Clone # 186107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
Leu29-Ser227
Accession # Q9JI58
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Rae‑1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB17582F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Rae-1

Rae-1 alpha, beta, gamma, δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Long Name
Retinoic Acid Early Transcript 1
Entrez Gene IDs
8480 (Human); 19368 (Mouse)
Alternate Names
dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1; Rae1; Rae-1

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Citations for Mouse Rae-1 Pan Specific Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor gammadelta are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection.
    Authors: Li Z, Zhang C, Zhou Z, Zhang J, Zhang J, Tian Z
    Infect. Immun., 2011-12-05;80(2):565-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
    Authors: Carayannopoulos LN, Barks JL, Yokoyama WM
    Biol. Reprod., 2010-05-19;83(3):404-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Inhibition of p-STAT3 enhances IFN-alpha efficacy against metastatic melanoma in a murine model.
    Authors: Kong LY, Gelbard A, Wei J
    Clin. Cancer Res., 2010-04-13;16(9):2550-61.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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