Mouse/Rat Butyrylcholinesterase/BCHE Antibody

Catalog #: AF9024
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF9024
AF9024-SP

Detection of Mouse Butyrylcholinesterase/BCHE by Western Blot.

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Citations (2)

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Mouse/Rat Butyrylcholinesterase/BCHE Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat Butyrylcholinesterase/BCHE in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Butyrylcholinesterase/BCHE
His28-Leu603
Accession # AAH99977

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Butyrylcholinesterase/BCHE by Western Blot. Western blot shows lysates of mouse heart tissue and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Polyclonal Antibody (Catalog # AF9024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Butyrylcholinesterase/BCHE at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Butyrylcholinesterase/BCHE in Mouse Brain. Butyrylcholinesterase/BCHE was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse/Rat Butyrylcholinesterase/BCHE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9024) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Butyrylcholinesterase/BCHE

Butyrylcholinesterase (BCHE) is a major acetylcholine hydrolyzing enzyme in the circulation (1). Although it is present in significant amounts (~3 mg/L) in human plasma, no endogenous physiological substrate has been described for this enzyme. It can degrade a large number of ester-containing compounds in addition to acylcholines. Thus, it is likely to play significant pharmacological and toxicological roles. It is thought to be involved in the pathological process of Alzheimer's disease (AD) by depleting acetylcholine. In contrast to ACHE, it attenuates amyloid fibril formation in vitro (2). BCHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance acetylcholine availability (3). Its involvement in a cholinergic anti-inflammatory pathway connect BCHE and ACHE with a possible marker of low-grade systemic inflammation observed in Type-2 diabetes, obesity, hypertension, coronary heart disease, and AD (4). BCHE can exist in monomeric and multimeric forms (1).

References

Darvesh, S. et al. (2003) Nat. Rev. Neuroscience 4:131.
Diamant, S. et al. (2006) Proc. Natl. Acad. Sci. USA 103:8628.
Campbell, V. A. and Gowran, A. (2007) Br. J. Pharm. 152:655.
Das, U. N. (2007) Med Sci Monit. 13:RA214.

Entrez Gene IDs

590 (Human); 12038 (Mouse)

Alternate Names

Acylcholine acylhydrolase; BCHE; Butyrylcholine esterase; Butyrylcholinesterase; CHE1; CHE1cholinesterase; Choline esterase II; cholinesterase 1; E1; EC 3.1.1.8; Pseudocholinesterase

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Citations for Mouse/Rat Butyrylcholinesterase/BCHE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Molecularly defined circuits for cardiovascular and cardiopulmonary control
Authors: A Veerakumar, AR Yung, Y Liu, MA Krasnow
Nature, 2022-06-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Systematic assessment of antibody selectivity in plasma based on a resource of enrichment profiles
Authors: C Fredolini, S Byström, L Sanchez-Ri, M Ioannou, D Tamburro, F Pontén, RM Branca, P Nilsson, J Lehtiö, JM Schwenk
Sci Rep, 2019-06-06;9(1):8324.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection