Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit

Name: Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit
Brand: R&D Systems
SKU: MB200
Rating: 5 (2 reviews)

Catalog #: MB200
14 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MB200

Ancillary Products Available

DY010: Sample Activation Kit 1

Mouse/Rat/Porcine/Canine TGF-beta 2 ELISA Standard Curve

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All TGF-beta 2 ELISAs

Product Details

Procedure

Citations (14)

FAQs

Supplemental Products

Reviews (2)

Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (25 uL), Cell Lysates (25 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)

Sensitivity

4.26 pg/mL

Assay Range

12.5 - 800 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Recombinant and natural mouse, rat, canine and porcine TGF-beta 2.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

Recovery

The recovery of TGF-beta 2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	92	80-102
Cell Lysates (n=2)	94	86-98
EDTA Plasma (n=4)	84	78-94
Heparin Plasma (n=4)	82	74-92
Serum (n=4)	98	85-114

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of TGF-beta 2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Scientific Data

N/A

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Mouse/Rat/Porcine/Canine TGF-beta 2 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TGF-beta 2

TGF-beta 2 (Transforming Growth Factor beta 2) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, 2 and 3 are encoded by separate genes, but are often called isoforms. They are highly pleiotropic cytokines that are proposed to act as cellular switches regulating processes such as immune function, proliferation and epithelial-mesenchymal transition (4-8). Mammalian TGF-beta 2 is secreted as a 395 amino acid (aa) proprotein that is processed by a furin-like convertase to generate an N-terminal latency-associated peptide (LAP, ~232 aa) and a C-terminal mature TGF-beta 2 (~112 aa) that remain associated via hydrogen bonding (9-13). Serine proteases such as plasmin, matrix metalloproteases, or thrombospondin-1, along with cofactors such as certain integrins, can dissociate LAP and release active TGF-beta 2 (11-14). In many types of cells, latent TGF-beta binding protein (LTBP) is covalently linked to the LAP homodimer prior to secretion. LTBP creates a large latent complex that is secreted, but may bind and localize to the extracellular matrix (10, 11). For TGF-beta isoforms, the latency components act as natural antagonists of TGF-beta activity, target TGF-beta to distinct tissues, and maintain a reservoir of TGF-beta (1). Mature mouse and rat TGF-beta 2 share 100% aa sequence identity, and share 97% aa identity with human, porcine, canine, equine and bovine TGF-beta 2.

TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and the TGF-beta RII type II ser/thr kinase receptor (15). In contrast, TGF-beta 1 and 3 have higher affinity for TGF-beta RII and do not require betaglycan (15). TGF-beta RII then phosphorylates and activates another ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (1, 6, 15, 16). Differences in structure of the prodomains and mature sequences of TGF-beta isoforms, and use of Smad-independent signaling pathways, allow for disparate actions observed in response to different TGF-beta isoforms and contexts (1-3, 12, 13, 15, 16).

Although many functions are overlapping, each TGF-beta isoform has some non-redundant functions. TGF-beta 2 plays a non-redundant role in human and mouse developmental heart valve remodeling, and mice with targeted deletions of TGF-beta 2 show defects in development of the cardiac system as well as lung, craniofacial, limb, eye, ear and urogenital systems (2, 7, 17, 28). TGF-beta 2 plays a unique positive regulatory role in hematopoiesis by enhancing Flt-3 signaling in hematopoietic progenitors (19). In humans, TGF-beta isoforms, especially TGF-beta 2, are identified as key factors in the progression of malignant glioma, gastric and ovarian cancer (8, 20-22). TGF-beta isoforms, particularly TGF-beta 2, suppress macrophage cytokine production and mucosal inflammatory responses in the developing intestine (23). In turn, macrophage LRP-1 can downregulate expression of TGF-beta 2 during vascular remodeling, which suppresses neo-intima formation (24).

Long Name:

Transforming Growth Factor beta 2

Entrez Gene IDs:

7042 (Human); 21808 (Mouse); 397084 (Porcine)

Alternate Names:

BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

50 µL Assay Diluent

Add 50 µL of Assay Diluent to each well.

50 µL Standard, Control, or Sample

Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

100 µL Conjugate

Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
Aspirate and wash 4 times.

100 µL Substrate Solution

Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

100 µL Stop Solution

Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat/Canine/Porcine TGF-beta 2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms
Authors: Tammam, BMH;Habotta, OA;El-Khadragy, M;Abdel Moneim, AE;Abdalla, MS;
Heliyon
Species: Rat
Sample Types: Tissue Homogenates
alpha1A Adrenoreceptor blockade attenuates myocardial infarction by modulating the integrin-linked kinase/TGF-beta/Smad signaling pathways
Authors: NM Alrasheed, RB Alammari, TK Alshammari, MA Alamin, AO Alharbi, AS Alonazi, AF Bin Dayel, NM Alrasheed
BMC cardiovascular disorders, 2023-03-24;23(1):153.
Species: Rat
Sample Types: Serum
Regenerative Effect of Mesenchymal Stem Cell on Cartilage Damage in a Porcine Model
Authors: Lin, S;Panthi, S;Hsuuw, Y;Chen, S;Huang, M;Sieber, M;Hsuuw, Y;
Biomedicines
Species: Porcine
Sample Types: Serum
Fibrillin-1 mutant mouse captures defining features of human primary open glaucoma including anomalous aqueous humor TGF beta-2
Authors: MK Ko, JI Woo, JM Gonzalez, G Kim, L Sakai, J Peti-Peter, JA Kelber, YK Hong, JC Tan
Oncogene, 2022-06-23;12(1):10623.
Species: Mouse
Sample Types: Aqueous Humor
EZH2-mediated H3K27me3 promotes autoimmune hepatitis progression by regulating macrophage polarization
Authors: G Chi, JH Pei, XQ Li
International immunopharmacology, 2022-02-19;106(0):108612.
Species: Mouse
Sample Types: Cell Culture Supernates
Microglia-derived interleukin-10 accelerates post-intracerebral hemorrhage hematoma clearance by regulating CD36
Authors: Q Li, X Lan, X Han, F Durham, J Wan, A Weiland, RC Koehler, J Wang
Brain, Behavior, and Immunity, 2021-02-13;94(0):437-457.
Species: Mouse
Sample Types: Tissue Homogenates
Role of epidermal growth factor receptor inhibitor-induced interferon pathway signaling in the head and neck squamous cell carcinoma therapeutic response
Authors: SP Korpela, TK Hinz, A Oweida, J Kim, J Calhoun, R Ferris, RA Nemenoff, SD Karam, ET Clambey, LE Heasley
Journal of Translational Medicine, 2021-01-23;19(1):43.
Species: Mouse
Sample Types: Cell Culture Supernates
Analysis of structural components of decellularized scaffolds in renal fibrosis
Authors: R Zhang, J Jiang, Y Yu, F Wang, N Gao, Y Zhou, X Wan, Z Wang, P Wei, J Mei
Bioactive materials, 2021-01-16;6(7):2187-2197.
Species: Rat
Sample Types: Cell Culture Supernates
GL261 luciferase-expressing cells elicit an anti-tumor immune response: an evaluation of murine glioma models
Authors: VE Sanchez, JP Lynes, S Walbridge, X Wang, NA Edwards, AK Nwankwo, HP Sur, GA Dominah, A Obungu, N Adamstein, PK Dagur, D Maric, J Munasinghe, JD Heiss, EK Nduom
Sci Rep, 2020-07-03;10(1):11003.
Species: Mouse
Sample Types: Cell Culture Supernates
De novo design of functional zwitterionic biomimetic material for immunomodulation
Authors: B Li, Z Yuan, P Jain, HC Hung, Y He, X Lin, P McMullen, S Jiang
Sci Adv, 2020-05-29;6(22):eaba0754.
Species: Mouse
Sample Types: Cell Culture Supernates

Evaluation of Neurosecretome from Mesenchymal Stem Cells Encapsulated in Silk Fibroin Hydrogels
Authors: Y Martín-Mar, L Fernández-, MH Sanchez-Re, N Marí-Buyé, FJ Rojo, J Pérez-Rigu, M Ramos, GV Guinea, F Panetsos, D González-N
Sci Rep, 2019-06-19;9(1):8801.
Species: Mouse
Sample Types: Cell Culture Supernates

TGF-?3 Promotes MUC5AC Hyper-Expression by Modulating Autophagy Pathway in Airway Epithelium
Authors: Y Zhang, H Tang, X Yuan, Q Ran, X Wang, Q Song, L Zhang, Y Qiu, X Wang
EBioMedicine, 2018-07-08;0(0):.
Species: Mouse
Sample Types: BALF

TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.
Authors: Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K
Nat Commun, 2015-02-19;6(0):6329.
Species: Mouse
Sample Types: Cell Culture Supernates

Resolvin D1 and lipoxin A4 improve alveolarization and normalize septal wall thickness in a neonatal murine model of hyperoxia-induced lung injury.
Authors: Martin C, Zaman M, Gilkey C, Salguero M, Hasturk H, Kantarci A, Van Dyke T, Freedman S
PLoS ONE, 2014-06-03;9(6):e98773.
Species: Mouse
Sample Types: Tissue Homogenates