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Mouse/Rat/Chicken Pax6 Antibody

Catalog #: MAB1260
6 Citations Datasheet / COA / SDS

Discontinued Product

MAB1260 has been discontinued.
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Pax6 in Rat Cortical Stem Cells.
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Product Details
Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat/Chicken Pax6 Antibody Summary

Species Reactivity
Mouse, Rat, Chicken
Specificity
Recognizes chicken, mouse, and rat Pax6.
Source
Monoclonal Mouse IgG1 Clone # PAX6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant chicken Pax6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Immunohistochemistry
Ericson, J. et al. (1997) Cell 90:169; Ericson, J. et al. (1992) Science 256:1555; Zhang, X.-M. et al. (2001) Dev. Biol. 233:271.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Pax6 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Pax6 in Rat Cortical Stem Cells. Pax6 was detected in immersion fixed undifferentiated rat cortical stem cells using Mouse/Rat/Chicken Pax6 Monoclonal Antibody (Catalog # MAB1260) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax6

Pax6 is a highly conserved transcription factor essential for the development of tissues including the eyes, central nervous system and endocrine glands of vertebrates and invertebrates (1-4). It is a key regulator that is required for normal islet development.

References
  1. Kumar, J.P. (2001) Nat. Rev. Genet. 2:846.
  2. Simpson, T.I. and D.J. Price (2002) Bioessays 24:1041.
  3. Schuurmans, C. and F. Guillemot (2002) Curr. Opin. Neurobiol. 12:26.
  4. Dohrman, C. et al. (2000) Mech. Dev. 92:47.
Long Name
Paired Box Gene 6
Entrez Gene IDs
5080 (Human); 18508 (Mouse); 25509 (Rat)
Alternate Names
keratitis); MGC17209; Oculorhombin; paired box 6; paired box protein Pax-6; Pax6

Product Datasheets

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Citations for Mouse/Rat/Chicken Pax6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Complement C5aR1 Signaling Promotes Polarization and Proliferation of Embryonic Neural Progenitor Cells through PKC?
    Authors: LG Coulthard, OA Hawksworth, R Li, A Balachandr, JD Lee, F Sepehrband, N Kurniawan, A Jeanes, DG Simmons, E Wolvetang, TM Woodruff
    J. Neurosci., 2017-04-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Two hypomorphic alleles of mouse Ass1 as a new animal model of citrullinemia type I and other hyperammonemic syndromes.
    Authors: Perez CJ, Jaubert J, Guenet JL, Barnhart KF, Ross-Inta CM, Quintanilla VC, Aubin I, Brandon JL, Otto NW, DiGiovanni J, Gimenez-Conti I, Giulivi C, Kusewitt DF, Conti CJ, Benavides F
    Am. J. Pathol., 2010-08-19;177(4):1958-68.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Palmitate affects insulin receptor phosphorylation and intracellular insulin signal in a pancreatic alpha-cell line.
    Authors: Piro S, Maniscalchi ET, Monello A
    Endocrinology, 2010-06-23;151(9):4197-206.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The Smo/Smo model: hedgehog-induced medulloblastoma with 90% incidence and leptomeningeal spread.
    Authors: Hatton BA, Villavicencio EH, Tsuchiya KD, Pritchard JI, Ditzler S, Pullar B, Hansen S, Knoblaugh SE, Lee D, Eberhart CG, Hallahan AR, Olson JM
    Cancer Res., 2008-03-15;68(6):1768-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Loss of patched and disruption of granule cell development in a pre-neoplastic stage of medulloblastoma.
    Authors: Oliver TG, Read TA, Kessler JD, Mehmeti A, Wells JF, Huynh TT, Lin SM, Wechsler-Reya RJ
    Development, 2005-04-20;132(10):2425-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
    Authors: Viti J, Gulacsi A, Lillien L
    J. Neurosci., 2003-07-02;23(13):5919-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Control

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