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Mouse/Rat Draxin Antibody

Catalog #: AF6149
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6149
AF6149-SP
Detection of Rat Draxin by Western Blot.
2 Images
Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Draxin Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Draxin in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Draxin is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Draxin
Gly26-Ile343
Accession # Q6PAL1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat Draxin antibody by Western Blot. View Larger

Detection of Rat Draxin by Western Blot. Western blot shows lysates of rat embryonic hippocampal glial cells. PVDF Membrane was probed with 1 µg/mL of Mouse/Rat Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Draxin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Draxin in Mouse Embryo. Draxin was detected in immersion fixed frozen sections of mouse embryo (E13) using Mouse Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cells in the midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Draxin

Draxin (Dorsal repulsive axon guidance protein; also neucrin) is a secreted, 58 kDa, presumable glycoprotein member of the Draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature mouse Draxin is 318 amino acids (aa) in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa 268-327). Mature mouse Draxin (aa 26-343) shares 80% aa identity with mature human Draxin, and 94% aa identity with mature rat Draxin.

Long Name
Dorsal Repulsive Axon Guidance Protein
Entrez Gene IDs
374946 (Human); 70433 (Mouse); 691317 (Rat)
Alternate Names
AGPA3119; C1orf187; chromosome 1 open reading frame 187; dorsal inhibitory axon guidance protein; Dorsal repulsive axon guidance protein; Draxin; FLJ34999; MGC117222; Neucrin; UNQ3119

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Citation for Mouse/Rat Draxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. DRAXIN regulates interhemispheric fissure remodelling to influence the extent of corpus callosum formation
    Authors: L Morcom, TJ Edwards, E Rider, D Jones-Davi, JW Lim, KS Chen, RJ Dean, J Bunt, Y Ye, I Gobius, R Suárez, S Mandelstam, EH Sherr, LJ Richards
    Elife, 2021-05-04;10(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot

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