Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody

Catalog #: PPS027
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PPS027

Detection of GABAAR alpha 5 C-Terminus by Western Blot.

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Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Mouse and rat ~55 kDa GABA_A R alpha 5 subunit, C-Terminus

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Fusion protein from the cytosolic loop of the rat GABA_A R alpha 5 subunit, C-Terminus

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of GABA_AR alpha 5 C-Terminus by Western Blot. Western blot of 7 μg of rat hippocampus showing specific immunolabeling of the ~55 kDa alpha 5 subunit of the GABA_AR.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 5

GABA_A ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA_A receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABA_A receptors have some combination of alpha, beta and gamma subunits. The rat alpha 5 isoform is a 439 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 25 - 232). The alpha 5 subunit is unusual in that it contains an N-terminal histidine which renders it very sensitive to zinc. It is also associated with extrasynaptic sites and seems to mediate tonic inhibition in hippocampal neurons.

References

Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
Caraiscos, V.B. et al. (2004) Proc. Natl. Acad. Sci. USA 101:3662.
Fisher, J.L. (2002) Neuropharmacology 42:922.
Loebrich, S. et al. (2006) EMBO J. 25:987.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-5 Polypeptide

Entrez Gene IDs

2558 (Human); 110886 (Mouse); 29707 (Rat)

Alternate Names

GABA A R alpha 5; GABA(A) receptor subunit alpha-5; GABAAR alpha 5; GABAARa5; GABRA5; gamma-aminobutyric acid (GABA) A receptor, alpha 5; gamma-aminobutyric acid receptor subunit alpha-5; MGC138184

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Citation for Mouse/Rat GABA-A R alpha 5 C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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