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Mouse/Rat Notch-1 Antibody

Catalog #: AF1057
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1057
AF1057-SP
Notch‑1 in Rat Cortical Stem Cells.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Notch-1 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects rat Notch‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant rat Notch-2 and recombinant mouse Notch-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Notch‑1
Arg20-Glu488 (Ala208Thr, Asp334Glu)
Accession # Q07008
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Notch‑1 Fc Chimera (Catalog # 1057-TK)
Flow Cytometry
2.5 µg/106 cells
Rat cortical stem cells
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 μg/mL of Goat Anti-Mouse/Rat Notch-1Antibody (Catalog # AF1057) will block 50% of the binding of 200 ng/mL of RecombinantRat Jagged 1 Fc Chimera (Catalog # 599-JG)to immobilized Recombinant Rat Notch-1 Fc Chimera (Catalog # Catalog # 1057-TK) coated at5 μg/mL (100 μL/well). At 20 μg/mL, this antibody will block >80% of thebinding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Notch-1 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Notch‑1 in Rat Cortical Stem Cells. Notch-1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Mouse/Rat Notch-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1057) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry Notch-1 antibody in Mouse Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Notch‑1 in Mouse Cortical Stem Cells. Notch-1 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Mouse/Rat Notch-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1057) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry View Larger

Notch‑1 in C2C12 Mouse Cell Line. Notch‑1 was detected in immersion fixed C2C12 mouse myoblast cell line using Goat Anti-Mouse/Rat Notch‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1057) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Notch-1 antibody in Rat Embryo by Immunohistochemistry (IHC-P). View Larger

Notch‑1 in Rat Embryo. Notch-1 was detected in immersion fixed paraffin-embedded sections of rat embryo (13 d.p.c.) using Goat Anti-Mouse/Rat Notch-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1057) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to labeling is in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Notch-1

Rat Notch-1 is a 300 kDa, type I transmembrane glycoprotein involved in a number of early-event developmental processes (1). In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The molecule is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1705 aa extracellular region, a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain (2). The large Notch-1 extracellular domain has 36 EGF‑like repeats followed by three notch/Lin-12 repeats. Of the 36 EGF‑like repeats, the 11th and 12th EGF‑like repeats have been shown to be both necessary and sufficient for binding the ligands Delta and Serrate, in Drosophila (3). The Notch-1 cytoplasmic domain contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The Notch-1 receptor undergoes post-translational proteolytic cleavage by a furin-like enzyme to form a heterodimer of the 1635 aa ligand binding extracellular region and the 877 aa transmembrane protein (4). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme and the Presenilin-dependent gamma -secretase results in the release of the Notch intracellular domain (NCID) which translocates into the nucleus where it functions as a transcription activator to initiate transcription of Notch-responsive genes (5). An alternative Notch signaling pathway that is mediated by the full-length form of Notch that has not been cleaved by the furin-like enzyme has also been reported (6). The rat Notch-1 extracellular domain shows 86% and 97% aa identity to human and mouse Notch-1 extracellular domains respectively. It also exhibits 56% and 50% aa identity with rat Notch-2 and Notch-3 extracellular domains, respectively.

References
  1. Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
  2. Weinmaster, G. et al. (1991) Development 113:199.
  3. Rebay, I. et al. (1991) Cell 67:687.
  4. Rogeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
  5. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
  6. Bush, G. et al. (2001) Dev. Biol. 229:494.
Entrez Gene IDs
4851 (Human); 25496 (Rat)
Alternate Names
EC 2.1.2.11; EC 3.4.21.68; hN1; Notch (Drosophila) homolog 1 (translocation-associated); notch 1Notch homolog 1, translocation-associated (Drosophila); Notch homolog 1, translocation-associated; Notch1; Notch-1; TAN1; TAN1neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1

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Citations for Mouse/Rat Notch-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions
    Authors: Maša Filipović, Darja Flegar, Alan Šućur, Dino Šisl, Inga Kavazović, Mariastefania Antica et al.
    Frontiers in Immunology
  2. OX40L/Jagged1 Cosignaling by GM-CSF–Induced Bone Marrow-Derived Dendritic Cells Is Required for the Expansion of Functional Regulatory T Cells
    Authors: Anupama Gopisetty, Palash Bhattacharya, Christine Haddad, Joseph C. Bruno, Chenthamarakshan Vasu, Lucio Miele et al.
    The Journal of Immunology
  3. Human-Induced Neural and Mesenchymal Stem Cell Therapy Combined with a Curcumin Nanoconjugate as a Spinal Cord Injury Treatment
    Authors: Pablo Bonilla, Joaquim Hernandez, Esther Giraldo, Miguel A. González-Pérez, Ana Alastrue-Agudo, Hoda Elkhenany et al.
    International Journal of Molecular Sciences
  4. Transplantation of Human-Fetal-Spinal-Cord-Derived NPCs Primed with a Polyglutamate-Conjugated Rho/Rock Inhibitor in Acute Spinal Cord Injury
    Authors: E Giraldo, P Bonilla, M Mellado, P Garcia-Man, C Rodo, A Alastrue, E Lopez, EC Moratonas, F Pellise, S ?or?evi?, MJ Vicent, V Moreno Man
    Cells, 2022-10-20;11(20):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Notch signaling pathway is a potential therapeutic target for extracranial vascular malformations
    Authors: RB Davis, K Pahl, NC Datto, SV Smith, C Shawber, KM Caron, J Blatt
    Sci Rep, 2018-12-20;8(1):17987.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling
    Authors: M Verma, Y Asakura, BSR Murakonda, T Pengo, C Latroche, B Chazaud, LK McLoon, A Asakura
    Cell Stem Cell, 2018-10-04;23(4):530-543.e9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Cell-Cell Contact Area Affects Notch Signaling and Notch-Dependent Patterning
    Authors: O Shaya, U Binshtok, M Hersch, D Rivkin, S Weinreb, L Amir-Zilbe, B Khamaisi, O Oppenheim, RA Desai, RJ Goodyear, GP Richardson, CS Chen, D Sprinzak
    Dev. Cell, 2017-03-13;40(5):505-511.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  8. Macrophage-dependent tumor cell transendothelial migration is mediated by Notch1/Mena(INV)-initiated invadopodium formation
    Sci Rep, 2016-11-30;6(0):37874.
    Species: Human, Mouse, Rat
    Sample Types: Cell Lysates, In Vivo, Whole Cells
    Applications: Bioassay, Neutralization, Western Blot
  9. Human stem/progenitor cells from bone marrow enhance glial differentiation of rat neural stem cells: a role for transforming growth factor beta and Notch signaling.
    Authors: Robinson AP, Foraker JE, Ylostalo J, Prockop DJ
    Stem Cells Dev., 2010-09-14;20(2):289-300.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Distinct stages of myelination regulated by gamma-secretase and astrocytes in a rapidly myelinating CNS coculture system.
    Authors: Watkins TA, Emery B, Mulinyawe S, Barres BA
    Neuron, 2008-11-26;60(4):555-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus
    Authors: Carrie J. Shawber, Lu Lin, Maria Gnarra, Mark V. Sauer, Virginia E. Papaioannou, Jan K. Kitajewski et al.
    Vascular Cell

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