Mouse/Rat PDX-1/IPF1 Antibody Summary
Met1-Arg284
Accession # P52946
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse/Rat PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX-1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line. PDX-1/IPF1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Mouse PDX‑1/IPF1 by Simple WesternTM. Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX-1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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PDX‑1/IPF1 in Mouse Pancreas. PDX-1/IPF1 was detected in perfusion fixed frozen sections of mouse pancreas using Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDX-1/IPF1
PDX-1 (Pancreas-duodenum homeobox 1; also IPF-1, IDX-1, STF-1, GSF and IUF-1) is a 42 kDa member of the IPF-1 subfamily, ANTP homeobox family of proteins. It is expressed in developing pancreas, plus pancreatic beta -cells, and duodenal crypt epithelium. In concert with HNF6, PDX-1 drives pancreatic endocrine cell formation by regulating the expression of neurogenin-3. In adult beta -cells, PDX-1 binds to PCIF-1 to regulate insulin production. Mouse PDX-1 is 284 amino acids (aa) in length (Accession # P52946). It contains one transactivation domain (aa 13‑73) a PBX1 P-P-M-W-K dimerization motif (aa 119‑124), and a DNA-binding homeodomain with an embedded NLS (aa 148‑206). Full-length mouse PDX-1 shares 94% and 88% aa identity with rat and human PDX-1, respectively.
Product Datasheets
Citations for Mouse/Rat PDX-1/IPF1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Identification of dynamic undifferentiated cell states within the male germline
Authors: HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs
Nat Commun, 2018-07-19;9(1):2819.
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Activated dormant stem cells recover spermatogenesis in chemoradiotherapy-induced infertility
Authors: Yang, SH;Zeng, YZ;Jia, XZ;Gu, YW;Wood, C;Yang, RS;Yang, JS;Yang, WJ;
Cell reports
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Structural and immunoendocrine remodeling in gut, pancreas and thymus in weaning rats fed powdered milk diets rich in Maillard reactants
Authors: J Dereke, E Ekblad, B Weström, C Erlanson-A, M Landin-Ols, I Sjöholm, M Hillman
Scientific Reports, 2022-03-08;12(1):4039.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Costus pictus D. Don leaf extract stimulates GLP-1 secretion from GLUTag L-cells and has cytoprotective effects in BRIN-BD11 beta -cells
Authors: Chinmai Patibandla, Zahidul Islam Khan, Louise MacGregor, Mark James Campbell, Steven Patterson
Journal of Ethnopharmacology
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Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA
Authors: C Miyagi-Shi, Y Nakashima, N Kobayashi, I Saitoh, M Watanabe, H Noguchi
Sci Rep, 2018-08-17;8(1):12341.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Expression of pancreatic and duodenal homeobox1 (PDX1) protein in the interior and exterior regions of the intestine, revealed by development and analysis of Pdx1 knockout mice
Authors: Haruo Hashimoto, Tsutomu Kamisako, Takahiro Kagawa, Seiki Haraguchi, Mika Yagoto, Ri-ichi Takahashi et al.
Laboratory Animal Research
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Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3beta in pancreatic beta-cells.
Authors: Semache M, Zarrouki B, Fontes G, Fogarty S, Kikani C, Chawki M, Rutter J, Poitout V
J Biol Chem, 2013-07-12;288(34):24825-33.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
Nature, 2012-10-07;491(7426):765-8.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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