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Mouse/Rat Plexin A3 Antibody

Catalog #: AF4075
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4075
AF4075-SP
Plexin A3 in Mouse Spinal Cord.
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Plexin A3 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse Plexin A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Plexin A1, rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, and rhPlexin D1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Plexin A3
Leu34-Asp150
Accession # P70208
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Plexin A3
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Plexin A3 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr). View Larger

Plexin A3 in Mouse Spinal Cord. Plexin A3 was detected in immersion fixed frozen sections of 13 d.p.c. embryonic mouse spinal cord using Goat Anti-Mouse Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry View Larger

Plexin A3 in Rat Brain (Cortex). Plexin A3 was detected in immersion fixed paraffin-embedded sections of rat brain (cortex) using Goat Anti-Mouse/Rat Plexin A3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4075) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuroglia. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin A3

Plexin A3 (also known as SEX) is a 220 kDa member of the Plexin A subfamily of semaphorin receptors. Mature mouse Plexin A3 is predicted to be an 1853 amino acid (aa) type I transmembrane glycoprotein that contains a 1202 aa extracellular domain (ECD) and a 629 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 34-473), three plexin-semaphorin-integrin (PSI) domains (aa 492-830), and four lg-like plexin-transcription factor (IPT) repeats (aa 841-1209). Plexin A3 interacts with neuropilins 1 and 2, and binds class 3 semaphorins. Over aa 35-150, mouse Plexin A3 is 99% and 97% aa identical to rat and human Plexin A3, respectively.

Entrez Gene IDs
55558 (Human); 18846 (Mouse); 309280 (Rat)
Alternate Names
HSSEXGENE; Plexin A3; plexin-4; plexin-A3; Plxn3; PLXN4; PLXNA3; Semaphorin receptor SEX; Sex chromosome X transmembrane protein of HGF receptor family 3; SEX; SEXPLEXIN-A3,6.3; XAP-6

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Citations for Mouse/Rat Plexin A3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. PLXNA1 and PLXNA3 cooperate to pattern the nasal axons that guide gonadotropin-releasing hormone neurons
    Authors: Roberto Oleari, Alessia Caramello, Sara Campinoti, Antonella Lettieri, Elena Ioannou, Alyssa Paganoni et al.
    Development
  2. Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement During Development
    Authors: JS Jung, KD Zhang, Z Wang, M McMurray, A Tkaczuk, Y Ogawa, R Hertzano, TM Coate
    J. Neurosci., 2019-06-17;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Neuropilin-2/Semaphorin-3F-mediated repulsion promotes inner hair cell innervation by spiral ganglion neurons
    Authors: Thomas M Coate, Nathalie A Spita, Kaidi D Zhang, Kevin T Isgrig, Matthew W Kelley
    eLife
  4. Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling.
    Authors: Demyanenko G, Mohan V, Zhang X, Brennaman L, Dharbal K, Tran T, Manis P, Maness P
    J Neurosci, 2014-08-20;34(34):11274-87.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Immunoprecipitation

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