Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody
Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody Summary
Ser156-Gln694
Accession # Q80W65
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse and Rat Proprotein Convertase 9/PCSK9 by Western Blot. Western blot shows lysates of mouse liver tissue and rat embryonic cortical neuron. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Proprotein Convertase 9/PCSK9 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Proprotein Convertase 9/PCSK9
PC9 (Proprotein convertase subtilisin type 9; also PCSK9 and NARC-1) is a 70‑74 kDa, secreted glycoprotein member of the proteinase K subfamily of subtilases. PC9 functions as a protease that cleaves proproteins at non-basic residues. It also binds to, and promotes, the degradation of LDLR, VLDLR and ApoER2. The mouse PC9 precursor is 694 amino acid (aa) in length, and contains a signal sequence, a 14 kDa, 121 aa prodomain (aa 35‑155), and a 56‑58 kDa, 539 aa mature region (aa 156‑694). The 58 kDa mature region arises from autocatalysis in the ER, and contains a subtilisin-like catalytic domain (aa 166‑432), a modified P-domain (aa 433‑529) and a C-terminal Cys-rich region (aa 530‑694). The prosegment and mature regions remain noncovalently associated. There is one additional PC9 isoform that shows an alternative start site 13 aa upstream of the standard site coupled to a deletion of aa 18‑20. Over aa 156‑694, mouse PC9 shares 93% and 79% aa identity with rat and human PC9, respectively.
Product Datasheets
Citations for Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Up-regulation of PCSK9 gene expression and diminished level of LDL-receptor in rat liver as a potential cause of post-lipectomy hypercholesterolemia
Authors: Agnieszka Dettlaff-Pokora, Elzbieta Sucajtys-Szulc, Tomasz Sledzinski
Molecular and Cellular Biochemistry
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Geranylgeranyl Isoprenoids and Hepatic Rap1a Regulate Basal and Statin-Induced Expression of PCSK9
Authors: Wang, Y;Tinsley, B;Spolitu, S;Zadroga, JA;Agarwal, H;Sarecha, AK;Ozcan, L;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Rosa26-LSL-dCas9-VPR: a versatile mouse model for tissue specific and simultaneous activation of multiple genes for drug discovery
Authors: D Pakalnišky, T Schönberge, B Strobel, B Stierstorf, T Lamla, M Schuler, M Lenter
Scientific Reports, 2022-11-10;12(1):19268.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Simple Western -
A multienzyme S-nitrosylation cascade regulates cholesterol homeostasis
Authors: CT Stomberski, NM Venetos, HL Zhou, Z Qian, BR Collison, SJ Field, RT Premont, JS Stamler
Cell Reports, 2022-10-25;41(4):111538.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: ICC, Western Blot -
Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice
Authors: EM Haberl, R Pohl, L Rein-Fisch, M Höring, S Krautbauer, G Liebisch, C Buechler
Lipids in Health and Disease, 2021-10-10;20(1):135.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels
Authors: T Rothgangl, MK Dennis, PJC Lin, R Oka, D Witzigmann, L Villiger, W Qi, M Hruzova, L Kissling, D Lenggenhag, C Borrelli, S Egli, N Frey, N Bakker, JA Walker, AP Kadina, DV Victorov, M Pacesa, S Kreutzer, Z Kontarakis, A Moor, M Jinek, D Weissman, M Stoffel, R van Boxtel, K Holden, N Pardi, B Thöny, J Häberle, YK Tam, SC Semple, G Schwank
Nature Biotechnology, 2021-05-19;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer
Authors: X Liu, X Bao, M Hu, H Chang, M Jiao, J Cheng, L Xie, Q Huang, F Li, CY Li
Nature, 2020-11-11;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Liver Pyruvate Kinase Promotes NAFLD/NASH in both Mice and Humans in a Sex-Specific Manner
Authors: KC Krishnan, RR Floyd, S Sabir, DW Jayasekera, PV Leon-Mimil, AE Jones, AA Cortez, V Shravah, M Péterfy, L Stiles, S Canizales-, AS Divakaruni, A Huertas-Va, AJ Lusis
Cell Mol Gastroenterol Hepatol, 2020-09-14;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
Authors: R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer
Genome Biol., 2018-09-19;19(1):137.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
?-Secretase Inhibition Lowers Plasma Triglyceride-Rich Lipoproteins by Stabilizing the LDL Receptor
Authors: K Kim, IJ Goldberg, MJ Graham, M Sundaram, E Bertaggia, SX Lee, L Qiang, RA Haeusler, D Metzger, P Chambon, Z Yao, HN Ginsberg, UB Pajvani
Cell Metab., 2018-03-22;0(0):.
Species: Mouse, Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Heparan sulfate proteoglycans present PCSK9 to the LDL receptor
Authors: C Gustafsen, D Olsen, J Vilstrup, S Lund, A Reinhardt, N Wellner, T Larsen, CBF Andersen, K Weyer, JP Li, PH Seeberger, S Thirup, P Madsen, S Glerup
Nat Commun, 2017-09-11;8(1):503.
Species: Hamster, Human
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: Western Blot -
A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing
Authors: Alireza Edraki, Aamir Mir, Raed Ibraheim, Ildar Gainetdinov, Yeonsoo Yoon, Chun-Qing Song et al.
Molecular Cell
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Consumption of salmon fishmeal increases hepatic cholesterol content in obese C57BL/6 J mice
Authors: Marit Hjorth, Atanaska Doncheva, Frode Norheim, Stine Marie Ulven, Kirsten Bjørklund Holven, Thomas Sæther et al.
European Journal of Nutrition
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Autocrine effects of PCSK9 on cardiomyocytes
Authors: Annemarie Wolf, Hanna Sarah Kutsche, Rolf Schreckenberg, Martin Weber, Ling Li, Susanne Rohrbach et al.
Basic Research in Cardiology
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AAV8-mediated overexpression of mPCSK9 in liver differs between male and female mice
Authors: Aimee E. Vozenilek, Cassidy M.R. Blackburn, Robert M. Schilke, Sunitha Chandran, Reneau Castore, Ronald L. Klein et al.
Atherosclerosis
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Platelet-Derived PCSK9 Is Associated with LDL Metabolism and Modulates Atherothrombotic Mechanisms in Coronary Artery Disease
Authors: Á Petersen-U, M Kremser, AK Rohlfing, T Castor, K Kolb, V Dicenta, F Emscherman, B Li, O Borst, D Rath, KAL Müller, MP Gawaz
International Journal of Molecular Sciences, 2021-10-16;22(20):.
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Hepatic Glucagon Signaling Regulates PCSK9 and Low-Density Lipoprotein Cholesterol
Authors: Stefano Spolitu, Haruka Okamoto, Wen Dai, John A. Zadroga, Erika S. Wittchen, Jesper Gromada et al.
Circulation Research
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Up-regulation of liver Pcsk9 gene expression as a possible cause of hypercholesterolemia in experimental chronic renal failure
Authors: Elzbieta Sucajtys-Szulc, Marek Szolkiewicz, Julian Swierczynski, Boleslaw Rutkowski
Molecular and Cellular Biochemistry
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Purified mouse Pcsk9 was used for immunoprecipitation (IP) validation.
5 ug AF3985 per IP incubation, works very well.
This Ab may be further diluted for each IP.
